CELLULAR PSYCHOPHARMACOLOGICAL MONITORING SYSTEM

该项目的长期目标是开发一种蜂窝 精神药理学监测系统的自由行为的啮齿动物， 包括大脑疾病的大鼠模型和转基因小鼠。 通过 开发新的设备和程序，细胞电生理学， 行为和微透析技术将融合成一个单一的， 计算机控制的装置。 在第一阶段，一个微处理器控制的微型液体开关- 透析液收集器/输送器，简称为微型阀，将 开发 这个装置将被放置在实验动物的头上 动物，并将连接到脑内植入的 微透析探针/微电极装置。 微型瓣膜将引导， 通过微透析探针，对照或药物溶液进入 记录神经元的细胞外环境。 由于 设备与神经元的接近程度、药物诱导的细胞 效果可以在一分钟内记录下来。此外，微型阀将 当透析液离开大脑时，将其收集到一个微型储液器中， 并将收集到的液体快速输送到神经化学分析中 系统 这些新颖的功能将开辟一个全新的方式， 确定细胞电生理和神经化学药物作用 在正常和异常的行为中，完整的大脑中。 拟议的微型阀将有助于获得新的见解， 脑的细胞电生理和神经化学基础 疾病，并有助于发现新药， 这些疾病的治疗。 建议的商业应用 拟议的多功能微型阀将为以下方面提供新的机会： 精神药理学研究 因此，预计 该设备将在美国的神经和精神药理学家中流行。 学术界 由于微型瓣膜将成为测定药物的新工具， 在大脑中的影响，它也预计该设备也将 广泛用于制药工业的体内实验室。

The long-term objective of this project is to develop a cellular psychopharmacological monitoring system for freely behaving rodents, including rat models of brain disorders and transgenic mice. By developing new devices and procedures, cellular electrophysiological, behavioral and microdialysis techniques will be fused into a single, computer-controlled apparatus. In Phase I, a microprocessor-controlled, miniature liquid switch - dialysate collector/transporter, briefly called as minivalve, will be developed. This device will be placed on the head of the experimental animal, and will be connected to an intracerebrally implanted microdialysis probe/microelectrode unit. The minivalve will direct, through the microdialysis probe, either control or drug solution into the extracellular environment of the recorded neurons. Due to the closeness of the device to the neurons, the drug-induced cellular effects can be recorded within a minute. Furthermore, the minivalve will collect the dialysates, as they leave the brain, into a microreservoir, and fast-transport the collected fluid into the neurochemical analysis system. These novel features will open up a completely new way for determining cellular electrophysiological and neurochemical drug effects in the intact brain, during both normal and abnormal behaviors. The proposed minivalve will help to obtain new insights into the cellular electrophysiological and neurochemical basis of brain disorders, and can contribute to the discovery of new drugs for the treatment of these diseases. PROPOSED COMMERCIAL APPLICATION The proposed multifunctional minivalve will offer new opportunities in psychopharmacological research. Therefore, it is anticipated that the device will be popular among neuro- and psychopharmacologists in the academia. Since the minivalve will be a new tool for determining drug effects in brain, it is also anticipated that the device will also be widely used in in vivo laboratories in the pharmaceutical industry.