DEVELOPMENT OF A RAPID ASSAY FOR VITAMIN D IN MILK

描述：维生素D对人体的生长和发育起着至关重要的作用。 保持骨骼健康。鲜奶和婴儿的强化 含有维生素D的配方奶粉在根除 儿童软骨病，然而，过量摄入会导致维生素D 醉酒等严重后果。因此，它势在必行。 牛奶样本和婴儿配方奶粉中含有正确的维生素D 标签上标明的浓度。然而，当前用于 维生素D的测定非常耗时。它需要 高技能的人员来执行实验，并需要几个 完成的天数。当他们收到报告时，他们的乳汁 产品已经运往市场并购买。 因此，本研究项目的目标是开发一种快速、 牛奶和婴儿配方奶粉中维生素D的灵敏、特异的检测 利用生物素和亲和素之间的高亲和力结合偶联到 辣根过氧化物酶建立酶联免疫吸附试验 (酶联免疫吸附试验)检测维生素D。因为酶联免疫吸附试验的敏感性取决于 使用的量热底物的检出限，调查者 建议使用化学发光酶联免疫吸附试验底物。这个 研究人员提出，敏感度至少可以提高3 比传统的量热分析高出几个数量级。具体的 这个第一阶段提案的目的是：1.开发一种快速和灵敏的 用非放射性测定法测定牛奶中维生素D浓度 化学发光和生物素-亲和素技术的结合，以及2. 建立一种快速、灵敏的非放射性维生素测定方法 婴儿配方奶粉中的D浓度 化学发光和生物素-亲和素技术。最终，一个简单的 商业化验试剂盒将被开发用于市场营销，将允许 牛奶和婴儿配方奶粉中维生素D含量的现场分析。 建议的商业应用：不可用

DESCRIPTION: Vitamin D plays an essential role for the growth and maintenance of healthy bones. Fortification of fresh milk and infant formula with vitamin D has played an important role in eradicating rickets in children, whereas, overfortification can cause vitamin D intoxication and other serious consequences. Thus, it is imperative that milk samples and infant formula have the correct vitamin D concentration as stated on the label. However, the current methods for the determination of vitamin D are very time consuming. It requires highly skilled personnel to perform the experiments and takes several days for completion. By the time they receive the reports, their milk products have already been shipped to the market and purchased. Therefore, the goal of this research project is to develop a rapid, sensitive, and specific assay for vitamin D in milk and infant formula utilizing high affinity binding between biotin and avidin coupled to horseradish peroxidase to develop an enzyme-linked immunosorbent assay (ELISA) for vitamin D. Because the sensitivity of ELISA depends on the detection limit for the calorimetric substrates used, the investigator proposes to utilize chemiluminescence ELISA substrates. The investigator proposes that the sensitivity can be increase at least 3 orders of magnitude over conventional calorimetric assay. The specific aims for this Phase I proposal are to:1. develop a rapid and sensitive non-radioactive assay to determine vitamin D concentration in milk using a combination of chemiluminescence and biotin-avidin technology, and 2. develop a rapid and sensitive non-radioactive assay to determine vitamin D concentration in infant formula using a combination of chemiluminescence and biotin-avidin technology. Eventually, a simple commercial assay kit will be developed for marketing that will allow an on-site analysis of vitamin D contents in milk and infant formula. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE