HOMEOBOX GENES AND THE MOLECULAR CONTROL OF LIMB PATTERN

描述：该提案代表了全球对 HOX基因在肢体发育过程中的表达。 塔宾认为， 特定HOX基因的表达控制阶段和区域特异性， 肢芽中的细胞增殖。 Tabin实验室以前的工作 已经表明，这些基因表达的嵌套模式沿着 肢芽的近端、远端和前后轴。 初步 实验中，Tabin描述了一种几乎 鸡体内的一整套基因。 在当前应用程序中，Tabin 建议比较错误表达个体的表型后果， HOX在肢体中的表达，并改变错误表达的时间，以确定是否 这些基因影响未分化细胞团的增殖或生长 已建立的骨骼元素。 将测定表型结果 从形态学和细胞增殖两方面来鉴定 受影响的特定细胞。 在第二个项目中，Tabin将使用差分 显示以鉴定HOX基因表达的下游靶标。 这些 目标将用于检查HOX基因的特异性， 具体目标。

DESCRIPTION: This proposal represents a global characterization of the expression of HOX genes during the development of the limb. Tabin argues that expression of specific hox genes controls stage and region specific cell proliferation in the limb bud. Previous work from the Tabin laboratory has shown that these genes are expressed in a nested patterns along the proximal distal and anterior posterior axis of the limb bud. In preliminary experiments, Tabin describes the isolation and expression of an almost complete set of these genes in chick. In the current application, Tabin proposes to compare the phenotypic consequences of misexpressing individual HOX in the limb and to alter the times of misexpression to determine whether these genes affect proliferation of the undifferentiated cell mass or growth of established skeletal elements. Phenotypic consequences will be assayed both morphologically and in terms of cell proliferation to identify the specific cells affected. In a second project Tabin will use differential display to identify downstream targets of HOX gene expression. These targets will be used to examine the specificity of HOX genes for there specific targets.