CELL-MATRIX INTERACTIONS IN AMPHIBIAN DEVELOPMENT

描述：这项建议来自一位知名的调查员，他已经 发表了该领域的优秀著作。他寻求继续 描述中胚层和细胞外之间的相互作用 在非洲爪哇早期胚胎中形成的基质。随着中胚层细胞在 胚胎，它们变得能动，并通过一种复杂和难以理解的机制， 移入胚胎内部。当他们这样做时，他们的行为会根据 它们是否会形成背侧、腹侧、前侧或后侧的结构。 向内生长的中胚层的底物之一是细胞外基质。 由胚腔顶端的细胞分泌。它是两个国家之间的互动 中胚层细胞和作为主题的细胞外基质糖蛋白 这项提议。其目的是1)确定细胞基质的作用 使用功能阻断试剂在原肠形成过程中的相互作用，2) 开发整合素激活位点的“记者”，将用于 监测受体激活的阶段和区域特异性变化，以及3) 看看中胚层基因的表达模式是否需要细胞-细胞外基质相互作用。

DESCRIPTION: This proposal is from an established investigator who has published excellent work in this field. He seeks to continue to characterize the interactions between the mesoderm and the extracellular matrix as it forms in early Xenopus embryos. As mesoderm cells form in the embryo, they become motile, and by a complex and ill-understood mechanism, move inside the embryo. As they do so, they behave differently according to whether they will form dorsal, ventral, anterior or posterior structures. One of the substrata of the ingressing mesoderm is the extracellular matrix secreted by the cells of the blastocoel roof. It is the interaction between mesoderm cells and the extracellular matrix glycoprotein that is the subject of this proposal. The aims are 1) to establish the roles of cell-matrix interactions during gastrulation using function-blocking reagents, 2) To develop "reporters" of sites of integrin activation that will be used to monitor stage and region-specific changes in receptor activation, and 3) to see if patterns of mesodermal gene expression require cell-ecm interaction.