FIBER OPTIC, IN VIVO CONFOCAL MICROSCOPY

本项目的目标是设计、构建和测试一种光纤 获得人体共焦反射光图像的光学导管 上皮组织在体内以几微米的分辨率在近真实的 时间 该器械的预期应用是帮助 上皮癌前病变的诊断和潜在筛查。 到 证明光纤的潜在临床优势 共焦成像，我们将评估我们的设备的能力， 测量体内组织形态，以帮助识别 子宫颈和口腔粘膜的癌前病变。 我们认为 该项目的成功完成将提供一个临床工具， 这将极大地提高对 口腔粘膜上皮癌前病变，宫颈，泌尿系 膀胱、结肠和其他器官的上皮细胞 癌 我们已经设计并测试了这个装置的原型 其使用单根光纤并且可商购获得， 宏观光学元件 然而，为了实现图像在 近视频率通过内窥镜，一个小，多纤维设备 其可以扫描整个上皮厚度。 我们 初步结果表明，这种装置是可行的。 目标 的建议是设计，建造和测试三个多纤维 解决三大工程进展的原型 需要获得整个上皮细胞的近视频速率图像 厚度与微米分辨率。 项目的具体目标 （1）设计一个多光纤共焦系统（prototype 1）， 可商购获得的宏观光学器件，以从 75-150 μ直径视场，2-3 μ横向分辨率 和5-7 μ轴向分辨率在1秒内从一个单一的焦平面 深度，平行于组织表面，位于约200 μ 在组织表面之下，（2）构建并测试该系统 正常志愿者的口腔粘膜，以及正常 和癌前子宫颈上皮的初步研究， 子宫颈上皮内瘤变（CN）;（3）研究 体内对比增强，（4）设计并测试1cm外 使用市售透镜的直径组件， 原型1;然而，在原型2中，待成像的组织平面 将不平行于组织表面，但将形成小的 与表面成一定角度，使图像平面中的细胞 从上皮表面到基底膜， 和（5）设计第三个原型，它将使用定制的光学系统， 远端部分的外径不大于3 mm，可与标准内窥镜联合使用 用于进入口腔粘膜和子宫以外部位的设备 子宫颈

The objective of this project is to design, construct and test a fiber optic catheter to obtain confocal reflected light images of human epithelial tissues in vivo with several micron resolution in near real time. The intended application of the device is to aid in the diagnosis, and potentially screening, of epithelial pre-cancers. To demonstrate the potential clinical advantages of fiber-optic confocal imaging, we will assess the capability of our device to measure tissue morphology in vivo to aid in the recognition of pre-cancers of the uterine cervix and oral mucosa. We believe that successful completion of this project will provide a clinical tool which could dramatically improve recognition and monitoring of epithelial precancers of the oral mucosa, uterine cervix, urinary bladder, colon, and other organs with high incidences of epithelial cancer. We have designed and tested a prototype of this device which uses a single optical fiber and commercially available, macroscopic optical components. However, to achieve images at near video rate through an endoscope, a small, multi-fiber device which can scan the entire epithelial thickness is required. Our preliminary results indicate that such a device is feasible. The goal of the proposal is to design, construct and test three multi-fiber prototypes to address the three major engineering advances required to achieve near viedeo rate images of the entire epithelial thickness with micron resolution. The specific aims of the project to: (1) design a multi-fiber confocal system (prototype1) which uses commercially available macroscopic optics to obtain images from a 75-150 mu diameter field of view with 2-3 mu lateral resolution and 5-7 mu axial resolution in 1 second from a single focal plane depth, parallel to the tissue surface, located approximately 200 mu beneath the tissue surface, (2) to construct, and test this system using phantoms, the oral mucosa of normal volunteers, and normal and pre-cancerous cervial epithelium in a pilot study of 10 patients ith cervical intra-epithelial neoplasia (CN), (3) to study methods of contrast enhancement in vivo, (4) to design and test a 1 cm outer diameter assembly of commercially available lenses to be used with prototype 1; however, in prototype 2, the tissue plane to be imaged will not be parallel to the tissue surface, but will form a small angle with the surface so that the cells in the image plane range from the surface of the epithelium to the basement membrane, and (5) to design a third prototype, which will use custom optics so that the outer diameter of the distal section is no greater than 3 mm and it can be used in conjuction with standard endoscopy equipment to access sites other than the oral mucosa and uterine cervix.