SIGNAL INTEGRATION IN THE MITRAL NEURON

描述(改编自申请人的摘要)：本报告的主要目的 建议通过直接测量来调查个体二尖瓣如何 神经元是按功能组织的，并将结果合并到一个 二尖瓣神经细胞的隔室模型。长期目标是使用 关于单个神经细胞的信息，作为 了解相互连接的神经元网络如何运行以控制 行为。从长远来看，我们所获得的关于正常程度的知识 行为的产生将帮助我们理解精神障碍，如 抑郁、吸毒或精神分裂症。了解单身的原因 神经元处理信息是理解大脑如何 行得通。对任何单个细胞的功能的完全了解并不是 还没有拿到。由于线性和非线性薄膜，地区性 分支过程的电学性质是复杂的，不可能 在没有空间分辨率测量的情况下进行预测。这些实验 将被用来详细描述一个样本神经元，即 大鼠嗅球内的二尖瓣神经元。我们将试着看看 兴奋性(来自嗅神经)和抑制性输入(来自)的处理 颗粒细胞)，以最直接的方式，通过光学监测膜 用细胞内电压敏感法测定多个部位的电势瞬变 染料。我们计划确定触发区的数量和位置，并 确定树突是否能够产生尖峰。我们要走了 从实验上检验这样的假设：除了根尖(主要) 枝晶反向繁殖，也出现在簇状和二次枝晶中 来影响突触的可塑性。我们还计划检验这一假设 单个神经元可以在功能上细分。如果为真，则此假设 将对功能复杂性有重要影响 单个神经元。

DESCRIPTION (Adapted from applicant's abstract): The main objective of this proposal is to investigate by direct measurement how individual mitral neurons are functionally organized and incorporate the results in a compartmental model of the mitral nerve cell. The long-term goal is to use the information about individual nerve cells as the basis for an understanding of how networks of interconnected neurons operate to control behavior. In the long run, the knowledge we obtain about how normal behavior is generated will help us understand mental disorders such as depression, drug addiction or schizophrenia. Understanding how single neurons process information is fundamental to understanding how the brain works. A complete understanding of any individual cell's function has not yet been obtained. Due to linear and nonlinear membranes, the regional electrical properties of branching processes are complex and impossible to predict in the absence of spatially resolved measurements. The experiments will be carried out to characterize in detail one exemplar neuron, the mitral neuron in the rat olfactory bulb. We will try to look at the processing of excitatory (from olfactory nerve) and inhibitory inputs (from granule cells), in the most direct way, by optical monitoring of membrane potential transients at many sites by using intracellular voltage sensitive dyes. We plan to determine the number and locations of trigger zones and to determine whether dendrites are capable of generating spikes. We are going to test experimentally the hypothesis that besides the apical (primary) dendrite the back propagation, also occurs in tuft and secondary dendrites to influence synaptic plasticity. We also plan to test the hypothesis that individual neurons can be functionally subdivided. If true, this postulate would have important implications for the functional complexity of individual neurons.