NON NEURONAL MECHANISMS CONTRIBUTING TO STROKE PATHOLOGY

描述(申请人摘要)：血管通透性增加是一种 缺血性脑损伤发生发展的重要因素 和浮肿。这项研究的目的是检查低氧对心脏的影响 血脑屏障的通透性特征。第一个目标是 目的：阐明不同负荷后血脑屏障通透性的功能变化 体外低氧和模拟再灌流的时间间隔。第二个是 确定钙依赖蛋白的定位和表达 细胞骨架蛋白、F-肌动蛋白与钙依赖的细胞黏附 分子，E-钙粘素，在低氧侮辱后。第三个目标是 通过进行核降解研究内皮细胞的凋亡 测量并检测bcl2的表达。这项提案的重点是 关于导致中风病理的非神经性机制。这些研究 将提供有关可能有助于 观察缺血后通透性的变化。

DESCRIPTION (Applicant's Abstract): Increased vascular permeability is an important factor contributing to the development of ischemic brain damage and edema. The goal of this research is to examine the effect of hypoxia on permeability characteristics of the blood-brain barrier. The first Aim is to elucidate the functional changes in BBB permeability after various intervals of hypoxia and simulated reperfusion in vitro. The second is to determine the localization and expression of the calcium-dependent cytoskeletal protein, F-actin, and the calcium-dependent cellular adhesion molecule, E-cadherin, after hypoxic insults. The third Aim is to investigate endothelial cell apoptosis by performing nuclear degradation measurements and examining BCL-2 expression. The focus of this proposal is on non-neuronal mechanisms that contribute to stroke pathology. The studies will provide new information about mechanisms that may contribute to permeability changes observed after ischemia.