HEPATIC MECHANISM FOR CONTROL OF FOOD INTAKE

描述（改编自申请人的摘要）：这是一个更新， 另外5年，给予肝脏对喂养的影响。 的 PI和他的同事已经完成了大量的工作与果糖 类似物，2，5-AM。 这种药物减少了肝脏中ATP的形成， 信号被传递到大脑，导致进食的开始。 的PI 现在建议完成必要的广泛研究，以证明 药物是否在肝脏中起作用以影响进食 三种其他抗代谢物：哇巴因、乙硫醚和巯基乙酸盐。 在相关的实验中，将给予标记药物， 分析肾脏、附睾脂肪和肌肉的摄取。 在第二个系列的实验中，门静脉内和肝内 将2，5-AM的输注与迷走神经的肝分支进行比较 完整或切割。目的是确定2，5-AM引起的进食是否 由在迷走神经的几个分支中行进的肝信号引起，或 还有肝床的几个器官 第三组实验 将使用两种方法，cFOS诱导和局部脑葡萄糖 利用标记的2-脱氧葡萄糖，以确定哪些区域的 下脑干被2，5-AM激活。

DESCRIPTION (Adapted from applicant's abstract): This is a renewal for 5 additional years a grant on the hepatic influences upon feeding. The PI and his colleagues have completed extensive work with the fructose analogue, 2,5-AM. The drug reduces ATP formation in the liver and a signal is relayed to the brain that causes initiation of eating. The PI now proposes to complete the extensive studies necessary to demonstrate that a drug does or does not act in the liver to influence eating with three other antimetabolites: Ouabain, ethionine and mercaptoacetate. In related experiments, labelled drug will be given and the liver, brain, kidneys, epididymal fat and muscle analyzed for uptake. In a second series of proposed experiments, intraportal and intrahepatic infusions of 2,5-AM will be compared with the hepatic branch of the vagus intact or cut. The purpose is to determine if 2,5-AM-elicited eating is caused by hepatic signals travelling in several branches of the vagus or else in several organs in the hepatic bed. A third series of experiments will use two methods, cFOS induction and local cerebral glucose utilization of labelled 2-deoxyglucose, to ascertain which areas of the lower brainstem are activated by 2,5-AM.