STRUCTURE/FUNCTION OF TRANS GOLGI NETWORK

描述：豪厄尔博士建议表征蛋白质所需的 从trans-Goli网络（TGN）出芽的胞吐囊泡，并解剖 它们在萌芽过程中的作用： TGN膜上的组分，出芽和囊泡的脱壳。 她的假设是细胞外囊泡的外壳结构 从TGN出芽的是花边状的TGNC，p62和p200将 成为这件外套的一部分。 具体目标1是生化解剖 p62复合物和p200在胞吐囊泡出芽中的作用 体外和体内。 具体目标2是p62的分子分析， p200。 具体目标3是定义胞吐囊泡外壳结构。 具体目标4是确定所需的附加组件， TGN囊泡出芽。

DESCRIPTION: Dr. Howell proposes to characterize proteins required for exocytic vesicle budding from the trans-Goli network (TGN) and to dissect their function in the steps of the budding process: recruitment of coat components to the TGN membrane, budding and uncoating of the vesicles. Her hypothesis is that the coat structure used in exocytic vesicle budding from the TGN is the lace-like TGNC, and that p62 and p200 will be components of this coat. Specific Aim 1 is biochemical dissection of the p62 complex and p200 function in exocytic vesicle budding in vitro and in vivo. Specific Aim 2 is molecular analysis of p62 and p200. Specific Aim 3 is defining the exocytic vesicle coat structure. Specific Aim 4 is identification of additional components required for TGN vesicle budding.