INTEGRATED EM-IMMUNOLABELING INSTRUMENTATION

10个主要用户要求资金提供综合仪器，这些仪器将提供在光学和电子显微镜水平上进行最先进的冷冻固定和免疫标签的能力。这些仪器包括一个Leica Ultracut UCT切片机与FSC冷冻附着。硅藻金刚石刀，爱德华兹自动306高真空沉积系统配置的电子显微镜，Bal-Tec喷射冻结装置和Bal-Tec冷冻替代单元。该仪器将被放置在由细胞和结构生物学系和癌症中心共同赞助的中央电磁设备中。他们将为设备提供翻新的空间，聘请荷兰乌得勒支大学的Jan Slot博士为我们已经熟练掌握显微切开术的科学家提供培训，并提供仪器的持续护理。这10位研究人员正在解决各种生物学问题，每个人都将受益于通过应用快速冷冻固定技术来提高他们的光学和电子显微镜的质量。他们的项目都需要em水平的免疫定位，制备和标记德康超薄冷冻切片的能力将加强他们NIH资助的研究。重要的是，该仪器将促进健康科学中心许多其他研究人员的研究，他们需要em免疫标记和使用各种形态学技术来研究保存良好的细胞和组织。高真空沉积系统是制备网格、涂层/阴影分子和大分子复合物的必要条件，以获得最佳的电子显微镜可视化。健康科学中心目前还没有类似的仪器。这个SGI的科学家小组有大学互动的历史，这一背景很好地说明了所要求的仪器的成功共享。

Ten major users request funds to provide integrated instrumentation that will provide the ability to carry out state-of-the-art cryo-fixation and immunounolabeling at both the level of light and electron microscopy. These instruments include a Leica Ultracut UCT microtome with an FSC cryo-attachment. Diatome diamond knives, an Edwards auto 306 high vacuum deposition system configured for electron microscopy, a Bal- Tec Jet Freeze device and a Bal-Tec Freeze substitution unit. This instrumentation will be placed in a central EM-facility that is co- sponsored by the Department of Cellular and Structural Biology and the Cancer Center. They will provide renovated space for the equipment, hire Dr. Jan Slot of the University of Utrecht in the Netherlands to provide training to our scientists already skilled in microtomy and provide for the continued care of the instruments. The ten investigators are addressing various biological questions and each would benefit from improving the quality of their light and electron microscopy by application of rapid freeze fixation techniques. Their projects all require immunolocalization at the EM-level and the ability to prepare and label Tokuyasu-ultrathin cryo sections will enhance their NIH funded research. Importantly this instrumentation will facilitate the research of many other investigators in the Health Sciences Center who require EM-immunolabeling and use of well preserved cells and tissues for study using a variety of morphological techniques. The high vacuum deposition system is necessary to prepare grids and coat/shadow molecules and macromolecular complexes for optimal visualization with the electron microscope. Comparable instruments are not currently available at the Health Sciences Center. The group of scientist on this SGI has a history of collegial interactions, a background that argues well for the successful sharing of the instrumentation requested.