14-3-3 PROTEIN FUNCTION IN CELL GROWTH AND MOTILITY

描述：本提案的重点是了解 14-3-3信号分子。 主要目标是建立14-3-3 特异性结合丝氨酸磷酸化蛋白质， 调节细胞增殖、分化和 细胞运动性 这一假设将通过三个具体的测试 实验目的 第一个是评估14-3-3的能力， 在体内结合特异性磷酸丝氨酸基序（通过使用突变体 Raf-1激酶的形式）。 第二个目标是审查 14-3-3在Raf-1介导的信号转导中的作用。 14-3-3抑制剂对非洲爪蟾胚胎发育的影响 第三个目标将集中在研究14-3-3在 细胞活性聚合和细胞运动的调节 成纤维细胞 希望这些实验的结果 确定14-3-3信号分子在细胞生长中的重要性 规范与发展。

DESCRIPTION: This proposal centers on understanding the function of the 14-3-3 signaling molecule. The major goal is to establish that 14-3-3 specifically binds to serine-phosphorylated proteins and helps to assemble signaling complexes that regulate cell proliferation, differentiation, and cell motility. This hypothesis will be tested through three specific experimental aims. The first will be to assess the ability of 14-3-3 to bind to specific phosphoserine motifs in vivo (through the use of mutant forms of the Raf-1 kinase). The second aim focuses on examining the role of 14-3-3 in Raf-1-mediated signal transduction through an analysis of the effects of specific 14-3-3 inhibitors on Xenopus laevis embryo development. The third aim will then center on examining the role of 14-3-3 in the regulation of acting polymerization and cell motility in cultured fibroblasts. The expectation is that the results of these experiments will establish the importance of the 14-3-3 signaling molecules in cell growth regulation and development.