CHEMOTAXIS AND PATHOGENESIS OF TREPONEMA DENTICOLA

本研究旨在研究齿垢密螺旋体的趋化性。T. 齿垢是一种革兰氏阴性口腔螺旋体， 与牙周病有关。 以前的研究已经认识到 运动性在允许细菌穿透 牙周组织并迁移到有利于细菌的部位] 增殖 这项资助申请提出，T。 齿垢是由趋化系统控制的，目的是研究 趋化基因和趋化行为。齿垢 总结 以下是拟议的实验：1）趋化性基因将 使用新分离的cheA PCR鉴定、克隆和测序 片段作为探针; 2）趋化性突变体将基于 关于插入基因破坏和同源重组;以及3） 将使用趋化性测定来表征趋化性突变体 在实验室里开发的。 从这些研究中学到的知识 可以深入了解T. 牙垢和其他螺旋体相关疾病。

This research aims to study chemotaxis of Treponema denticola. T. denticola, a motile gram negative oral spirochete, was found to be associated with periodontal diseases. Previous studies have recognized the role motility plays in allowing the bacterium to penetrate periodontal tissues and migrate to sites favorable to bacteria] proliferation. This grant application proposes that the motility of T. denticola is controlled by a chemotaxis system and aim to study the chemotaxis genes and chemotactic behavior of T. denticola. Summarized here are the proposed experiments: 1) chemotaxis genes will be identified, cloned and sequenced using a newly isolated cheA PCR fragment as the probe; 2) chemotaxis mutants will be constructed based on insertional gene disruption and homologous recombination; and 3) the chemotaxis mutants will be characterized using the chemotaxis assays developed in the laboratory. The knowledge learned from these studies could give insights into the molecular mechanisms of pathogenesis of T. denticola and other spirochete-related diseases.