ROLE FOR FKBP12 IN GNRH SIGNALING

描述：（改编自申请人的描述）本研究基于 假设在促性腺激素细胞中，FKBP 12可能是一种细胞蛋白， IP 3受体的调节蛋白，并通过GnRH信号传导控制。 该提案的主要目标是评估FKBP 12在 调节IP 3受体功能和GnRH诱导的LH分泌。 的 研究建议量化FKBP 12对GnRH诱导的LH的贡献 使用体外和体内模型， IP 3-受体复合物的表达、组装或磷酸化是 由激素调节，改变对GnRH的敏感性。 该项目应提供有关GnRH刺激PKC的信息 导致钙从内质网释放的途径。 它将 使用药物FK 506来测试FKBP 12的作用。 FK 506是一款 广泛用于器官移植的免疫抑制药物。 因此，在本发明中， 关于其在生殖系统中的潜在作用的信息也将 获得。

DESCRIPTION: (Adapted from applicant's description) This research is based on the hypothesis that, in gonadotropes, a cellular protein, FKBP12 may be a regulatory protein for IP3 receptors and be controlled via GnRH signaling. The broad goal of the proposal is to evaluate the role of FKBP12 in the regulation of IP3 Receptor function and GnRH-induced LH secretion. The study proposes to quantify the contribution of FKBP12 to GnRH-induced LH secretion using in vitro and in vivo models and to determine whether expression, assembly, or phosphorylation of the IP3-Receptor complex is regulated by hormones that alter sensitivity to GnRH. The projects should provide information about the GnRH stimulated PKC pathway leading to calcium release from the endoplasmic reticulum. It will use a drug, FK506, to test the role of FKBP12. FK506 is an immunosuppressive drug that is widely used in organ transplants. Thus, information about its potential role in the reproductive system would also be obtained.