Shear stimulated Brillouin microscopy for cell mechanobiology

本项目旨在开发细胞和组织微力学特性的非接触成像新技术，以回答细胞力学生物学的基本问题。基于布里渊光散射原理，该项目利用了射频锁定检测方案。该项目将为细胞局部刚度和压缩性的空间表征提供实时、高灵敏度、非接触式3D成像解决方案。这将巩固细胞力学生物学和疾病研究领域的知识进步，在这些领域中，细胞力学特性的微尺度变化导致细胞功能障碍和凋亡。

This project aims to develop novel technology for non-contact imaging of micro-mechanical properties in cells and tissues to answer fundamental questions of cell mechnanobiology. Based on principles of Brillouin light scattering, the project takes advantage of a radio-frequency lock-in detection scheme. The project will result in a real-time, high-sensitivity, non-contact 3D imaging solution for spatial characterisation of cell's local stiffness and compressibility. This will underpin the advancement of knowledge in the area of cell mechanobiology and the investigation of diseases, where microscale changes in cell mechanical properties lead to cell dysfunction and apoptosis.