High-performance yeast for sustainable liquid fuel

酵母（saccharomyces_ cerevisiae_）在生物乙醇生产中的碳利用效率遭受重大损失，因为生物体也产生甘油作为（废物）副产物来帮助维持其渗透平衡。这种低价值的废物使生物乙醇的产量降低了5%。其他人则试图解决这个问题，利用酵母生物工程来减少甘油的产量，并将更多的原料碳转向乙醇。该方法已经取得了部分成功，提高了碳效率并维持了细胞还原-氧化（REDOX）平衡。早期方法的局限性在于，转化为乙醇的工程代谢途径的中间体可以转化为其他细胞代谢物，因此无法实现完全的碳重定向效益。我们的创新方法为我们的竞争对手提供了关键优势。它同样会减少甘油的合成，而是通过一种不需要代谢的替代中间体将碳重定向到乙醇中，从而增强最大的碳利用效率和乙醇生产率。Ingenza从事酵母生物工程已经超过10年，开发了所有必要的专业知识，使技术工具和能力能够实现这个项目。我们的市场战略包括在与主要国际合作伙伴的现有技术许可关系下将项目产出商业化。我们将通过专利保护和合作伙伴的监管来保护我们的项目成果。项目投资的潜在回报对Ingenza和英国纳税人都具有重大价值。全球生物乙醇市场充满活力，（直到最近）复合年增长率为5.3%（2014-2019年），2019年全球使用量为2900万加仑。虽然我们认识到该技术在许多市场（例如饮料酒精）中存在替代机会，但预计到2024年，仅生物乙醇市场的价值就将达到796亿美元。项目技术的成功部署可以为Ingenza带来可观的回报，为未来的增长计划提供就业保障和投资。该项目将允许英国领先的工业生物技术开发商积极参与协同商业合作，提供颠覆性技术，扩大可持续液体燃料的使用，更广泛地推进英国生物经济，对气候变化和环境可持续性产生积极影响。

Carbon utilisation in bioethanol production by the yeast _Saccharomyces_ _cerevisiae_ suffers significant efficiency losses because the organism also produces glycerol as a (waste) co-product to help maintain its osmotic balance. This low value waste product compromises bioethanol yield by up to 5%. Others have tried to address this problem, employing yeast bioengineering to reduce glycerol production and redirect more feedstock carbon instead to ethanol. The approach has achieved partial success, increasing carbon efficiency and maintaining cellular reduction-oxidation (REDOX) balance. The limitation of earlier approaches is that the intermediates of the engineered metabolic pathway to ethanol can be converted to other cellular metabolites, such that the full carbon redirection benefits cannot be realised. Our innovative approach provides key advantages over that of our competitors. It will similarly reduce glycerol synthesis but instead redirects carbon to ethanol via an alternate intermediate which is not otherwise metabolised, thereby potentiating maximal carbon use efficiency and ethanol productivity gains.Ingenza has conducted yeast bioengineering for over 10 years, developing all necessary expertise, enabling technology tools and capabilities to deliver this project. Our go-to-market strategy involves the commercialisation of project outputs under an existing technology license relationship with a major international partner. We will safeguard our project outcomes through patent protection and the partner's policing. The potential return on project investment represents significant value for both Ingenza and UK taxpayers. The global bioethanol market is dynamic with (until recently) 5.3% CAGR (2014-2019) and usage in 2019 of 29 million gallons globally. While we recognise that there are alternative opportunities for the technology in many markets (e.g. beverage alcohol) the bioethanol market alone is expected to be valued at $79.6 billion by 2024\. Successful deployment of the project technology could secure significant returns for Ingenza, underpinning job protection and investment in future growth initiatives. The project will allow a leading UK developer of industrial biotechnology to aggressively participate in a synergistic commercial collaboration to deliver disruptive technology that expands the use of sustainable liquid fuels and advances the UK bioeconomy more broadly, to positively impact climate change and environmental sustainability.