GENETIC ANALYSIS OF MIDLINE PATTERNING IN ZEBRAFISH

诱导信号网络的性质，模式， 脊椎动物神经外胚层是发育生物学的中心问题。 下垫面对底板分异的诱导作用 脊索是一种信号事件的范例， 神经管的背腹轴。底板诱导是介导的 由音速刺猬，但仍有很多未知的机制， 其规定了底板单元的命运。我想了解一下 地板规范的基础上有了细胞和分子 斑马鱼（Danio rerio）突变体剑水蚤（cyc）的分析， 删除底板和其他腹侧神经外胚层细胞类型。 这项提议将检验cyc具有基本的 地板规范中的功能，可能介导了 Sonic Hedgehog..这项建议的目的是（1） 在野生型和cyc突变胚胎中表征底板前体 使用命运作图技术，和（2）从分子水平鉴定CYC 基因定位克隆。这些实验将提供关键的 关于野生型中中线细胞类型的规格的信息 胚胎，以及cyc在这个过程中所起的作用。

The nature of the inductive signaling networks that pattern the vertebrate neuroectoderm is a central problem in developmental biology. The induction of the floor plate differentiation by the underlying notochord is a paradigm of the signaling events that establish the dorsoventral axis of the neural tube. Floor plate induction is mediated by Sonic hedgehog, but much remains unknown about the mechanisms by which the floor plate cell fate is specified. I propose to learn about the basis of floor plate specification with a cellular and molecular analysis of the zebrafish (Danio rerio) mutant cyclops (cyc), which deletes the floor plate and other ventral neuroectodermal cell types. This proposal will test the hypothesis that cyc has an essential function in floor plate specification, perhaps mediating the inductive action of Sonic hedgehog.. The aims of this proposal are (1) to characterize floor plate precursors in wild-type and cyc mutant embryos using fate mapping techniques, and (2) to molecularly identify the cyc gene by positional cloning. These experiments will provide critical information about the specification of midline cell types in wild-type embryos, and the role that cyc plays in the process.