STABALIZED LIPID PLATFORMS FOR PROTEIN IMMOBOLIZATION

提出的研究目标是制备和表征新型聚合物磷脂双分子层组件作为蛋白质附着的底物。在双分子层表面固定化蛋白质受体对于开发利用结合蛋白作为生物选择性识别元件的新型生物传感器具有重要的实际应用价值。实现脂质双分子层作为蛋白质固定化和随后用作生物传感器的底物，需要在保持脂质双分子层低非特异性结合特性的同时，改善其化学和结构特性。这里要研究的策略是磷脂双分子层的侧向聚合，以及在表面上定向固定蛋白质。提出的研究将涉及新型系链平面聚合物磷脂双分子层的制备和表征。所制备的交联聚合物磷脂双分子层的稳定性将通过溶解度和光学测量来评估。聚合物脂双分子层随后将被用作定点蛋白固定化的底物。提出的研究将试图辨别在脂质双分子层交联和与底物的共价附着后，是否保留了流体脂质双分子层的低非特异性吸附特性。由此产生的组装应该是一个稳定的蛋白质-脂质组装，具有吸附蛋白质膜的定向呈现，最大限度地保留了蛋白质的结构和功能。

The goal of the proposed research is to prepare and characterize novel polymeric phospholipid bilayer assemblies as substrates for protein attachment. Immobilization of protein receptors on the bilayer surface has important practical applications for the development of new biosensors utilizing the bound protein as the biologically selective recognition element. The implementation of lipid bilayers as substrates for protein immobilization and subsequent use as biosensors requires that the chemical and structural properties be improved while maintaining the low nonspecific binding properties of lipid bilayer. The strategy to be investigated here is the lateral polymerization of the phospholipid bilayer in conjunction with site directed immobilization of proteins on the surface. The proposed research will involve preparation and characterization of novel tethered planar polymer phospholipid bilayers. The stability of the prepared cross- linked polymer phospholipid bilayers will then be assessed by solubility and optical measurements. The polymer lipid bilayers will subsequently be utilized as substrates for site directed protein immobilization. The proposed research will attempt to discern if the low nonspecific adsorption characteristic of fluid lipid bilayer are preserved upon cross-linking of the lipid bilayer and covalent attachment to the substrate. The resulting assembly should result in a stable protein- lipid assembly with an oriented presentation of the adsorbed protein film with maximum retention of protein structure and function.