PHOTOPHYSICS AND PHOTODYNAMIC EFFICIENCY OF HEMATOPORPHYRINS

该项目旨在改善光敏化的现状 用于肿瘤疾病的检测和光动力治疗。 由于血卟啉衍生物和Photofrin的价值较好， 记录，该项目将检查电子和荧光 HpD和Photofrin-II的三种已知组分的作用： 原卟啉、血卟啉和二血卟啉醚/酯。这些 将通过紫外/可见光检查三种香豆素， 激发/发射荧光分光光度法在无金属和 金属锌的形式。无金属和锌金属化的卟啉将 在无细胞的PBS（磷酸盐缓冲盐水; pH-7.2）中进行检查， 在含有PTK2细胞（大鼠袋鼠上皮肾）的PBS溶液中 细胞）。含有PTK2细胞的相应卟啉溶液将 也受到来自染料激光器2的光照射，以检查 无金属和金属化锌卟啉的光毒性。 本项目的具体目标是测试锌取代卟啉 针对HpD和Photofrin-II的已知组分的复合物。的影响 要检查的内容包括：1）。辨别及指派 电子跃迁。2）。荧光寿命的比较 和量子产率，3）。光杀伤的测量和比较 比率。HpD和Photofrin-II成分与其成分的比较 金属化的锌类似物是重要的，因为众所周知， 金属卟啉比金属卟啉更能发出荧光和磷光。 脱金属的对应物如果可以证明锌取代 例如，原卟啉表现出比 脱金属形式的原卟啉，该络合物可用作 癌细胞定位器如果这些锌卟啉 显示出上级细胞致敏性，它们可用作 光敏剂，作为HpD的前体或与HpD结合。

This projects seeks to improve the current state of photosensitization used in the detection and photodynamic therapy of neoplastic disease. Since the value of Hematoporphyrin Derative and Photofrin are well documented, this project will examine the electronic and fluorescent effects of three of the known components of HpD and Photofrin-II: protoporphyrin, hematoporphyrin, and dihematoporphyrin ether/ester. These three prophyrins will be examined by ultraviolet/visible and excitation/emission fluorescence spectrophotometry in the metal free and metallated Zinc forms. The metal free and Zinc metallated porphyrins will be examined in PBS (phosphate buffered saline; ph-7.2) free of cells and in PBS solutions containing PTK2 cells (rat kangaroo epithelial kidney cells). The respective porphyrin solutions containing the PTK2 cells will also be subjected to photoirradiation form a dye laser2 to examine the phototoxicity of the metal-free and metallated Zinc porphyrins. The specific aims of this project are to test Zinc substituted porphyrin complexes against known components of HpD and Photofrin-II. The effects to be examined include the following: 1). identification and assignment of electronic transitions. 2). a comparison of the fluorescence lifetime and quantum yields, 3). measurements and comparisons of the photokill ratios. The comparison of HpD and Photofrin-II components against their metallated Zinc analogues is important because it is well known that metalloporphyrins are better able to fluoresce and phosphoresce than their demetallated counterparts. If it can be shown that Zinc substituted protoporphyrin, for example, exhibits a superior fluorescence than does protoporphyrin in the demetallated form, this complex could be used as a localizer for cancer cells. If any one of these Zinc-porphyrins exhibited superior sensitization of cells, they could be used as photosentizers, either as precursors for or in conjunction with HpD.