PHTHALOCYANINE PHOTOSENDITIZER EFFICIENCY ON PTK2 CELLS

本项目的目的是测量荧光量子产率， 荧光寿命、三重态量子产率和三重态寿命 15种光敏剂，并将这些测量结果与 比较这十五种化合物的体外光灭活效率， PTK 2大鼠袋鼠上皮肾细胞中的光敏剂。 的 15种光敏剂是：酞菁（Pc），其二-和 四磺酸盐（PcDs，PcTs）; Pc，PcDs，PcTs的锌金属化络合物; 氯铝酞菁及其四磺酸盐（CIAIPc，CIAIPcTs）; 萘酞菁（NPc）、ZnNpc、脱镁叶绿酸甲酯a（Mppb）;单-L- 血卟啉IX 和Photofrin（PII），一种浓缩形式的HpD。 光物理 测量将在二甲基甲酰胺（DMF）中进行， 将在磷酸盐缓冲盐水中进行光细胞毒性研究 (PBS)在可能的情况下，或者对于二氢卟酚衍生物，在PBS-克列莫佛中。 光敏剂将被引入PTK 2大鼠袋鼠上皮细胞 24小时，暴露于适当波长的光， 一个Nd-YAG激光器，并评估每种复合物的光杀伤效率 利用台盼蓝试验测定细胞活力。这项实验旨在 提供一个预测光杀伤效率的模型， 光敏剂基于其物理参数。 这项研究代表了第一次全面研究的照片物理 行为和光细胞毒性效率的各种各样的 光敏剂，包括“新”和“旧”类光敏剂- 酞菁及其衍生物，叶绿素和二氢卟酚衍生物- Photofrin II。假设最好的光敏剂是 那些具有高量子产率的长寿命三重态的化合物， 氧浓度保持恒定。

The aim of this project is to measure the fluorescence quantum yield, fluorescence lifetime, triplet quantum yield and triplet lifetime for fifteen photosensitizers, and correlate these measurements with the comparative in vitro photoinactivation efficiency of these fifteen photosensitizers in PTK2 rat kangaroo epithelial kidney cells. The fifteen photosensitizers are: phthalocyanine (Pc), its di- and tetrasulfonate (PcDs,PcTs); zinc metallated complexes of Pc, PcDs, PcTs; chloroaluminum phthalocyanine and its tetrasulfonate (CIAIPc, CIAIPcTs); napthalocyanine (NPc), ZnNpc, methyl pheophorbide a (Mppb); mono-L- aspartyl chlorin E6 (MACE); tin etiopurpurin (SnEt); hematoporphyrin-IX and Photofrin (PII), a concentrated form of HpD. Photophysical measurements will take place in Dimethylformamide (DMF) and photocytotoxicity studies will take place in phosphate buffered saline (PBS), where possible, or in PBS- cremophor for the chlorin derivatives. Photosensitizers will be introduced to PTK2 rat kangaroo epithelial cells for twenty-four hours, exposed to light at the appropriate wavelength from a Nd-YAG laser, and the photokill efficiency of each complex assessed utilizing the trypan blue test for cell viability. This experiment seeks to provide a model for predicting the photokill efficiency of photosensitizers based on their photophysical parameters. This study represents the first comprehensive study of the photo physical behavior and the photocytotoxicity efficiency of a wide variety of photosensitizers, including "new" and "old" classes of photosensitizers- phthalocyanine and its derivatives, chlorophyll and chlorin derivatives- and Photofrin II. The hypothesis is that the best photosensitizers are those which possess a long-lived triplet state in high quantum yield when the oxygen concentration remains constant.