DETECTION OF MACROMOLECULES WITH QUANTUM DYES

描述（来自申请人摘要的逐字逐句）： 该项目是开发发光生物标志物， 在大环配体上官能化的镧系元素（III）络合物，“Quantum Dyes. " 以前的工作表明，功能化的铕（III）大环配合物 能够共价连接到生物物质上， 自由的时间选通发光测量。然而，对于许多实际 应用它们的发光强度必须增加。第一阶段工作 导致：1）合成含有多个Eu-大环的标记物，2） Eu-大环化合物发光的增强作用 能量转移供体或提供保护免于淬灭，和3）增强剂 发现促进了来自原型非官能化的发光 铽（III）-大环化合物。铕（III）和铽的同晶对 (III)官能化的发光大环将允许灵敏、精确、 比较杂交研究。 在第二阶段：1）细胞凋亡和DNA合成的敏感性测试将是 2）含有多个标记物的非特异性结合 Eu-大环将被最小化，3）官能化的铽（III）大环 将被合成并测试。4)初步工作将围绕如何 最大化来自铕（III）和 大环铽（III）; 5） 量子染料的检测。 拟定商业应用： 未提供拟议的商业应用。

DESCRIPTION (Verbatim from the applicant's abstract): The long-term goal of this project is the development of luminescent biomarkers containing lanthanide(III) complexes functionalized on macrocyclic ligands, "Quantum Dyes." Previous work has shown that functionalized europium(III) macrocyclic complexes are capable of covalent attachment to bio-substances and permit background free, time-gated luminescence measurements. However, for many practical applications their luminescence intensity had to be increased. The Phase I work resulted in: 1) The synthesis of markers containing multiple Eu-macrocycles, 2) Enhancement of the Eu-macrocycle luminescence by additives that act as energy-transfer donors or provide protection from quenching, and 3) An enhancer was found that promotes luminescence from the prototype non-functionalized terbium (III-macrocycle. An isomorphous pair of europium (III) and terbium (III) functionalized luminescent macrocycles will permit sensitive, precise comparative hybridization studies. In Phase II: 1) Sensitive tests for apoptosis and DNA synthesis will be developed, 2) The nonspecific binding of markers containing multiple Eu-macrocycles will be minimized, 3) Functionalized terbium(III) macrocycles will be synthesized and tested. 4) Preliminary work will be performed on how to maximize the luminescence from the combined use of europium (III) and terbium(III) macrocycles, and 5) Improvements to the instrumentation for detecting Quantum Dyes will be made. PROPOSED COMMERCIAL APPLICATION: Proposed commercial application not available.