RT-PCR /ELISA BASED METHOD FOR LEUKEMIA DIAGNOSIS

急性淋巴细胞白血病（ALL）是最常见的恶性肿瘤， 童年.儿童白血病的复发性染色体易位 用于诊断、预后和治疗的有用的细胞遗传学标志物 监测.例如，某些特定易位的存在， 包括t（1 ;19）、t（9;22）和其他定义了一组ALL患者， 治疗失败的风险更高。其他异常，包括 t（12;21）与一组ALL患者相关， 治疗失败的风险。因此，识别这些 在诊断时的异常是必要的，如果适当的治疗 就是给予我们提出了一种新的技术， 一次检测多个融合转录物。该方法还可以 用于定量研究融合蛋白的表达水平 成绩单。因此，开发这样一种检测试剂盒， 诊断.儿童ALL的预后和治疗。 拟定商业应用： 基于所提出的研究，可以开发分子诊断试剂盒。 该试剂盒可提供快速、准确、灵敏和定量的诊断 白血病特有的染色体易位此外，96井 ELISA平板形成试验可检出多达16种常见的 易位

Acute Lymphoblastic Leukemia (ALL) is the most common malignancy of childhood. Recurrent chromosome translocations in childhood leukemia are useful cytogenetic markers for diagnosis, prognosis and treatment monitoring. For example, the presence of certain specific translocations, including t(1 ;19), t(9;22) and others defines a group of ALL patients who are at a higher risk of treatment failure. Other abnormalities, including t(12;21), are associated with a group of ALL patients who are at a low risk of treatment failure. Therefore, identification of these abnormalities at the time of diagnosis is essential if appropriate therapy is to be given. We present a novel technology that will allow efficient detection of multiple fusion transcripts at a time. The method could also be used to quantitatively study the expression level of the fusion transcript. Develop such a test kit, therefore, could dramatically improve diagnosis. prognosis and treatment of childhood ALL. PROPOSED COMMERCIAL APPLICATION: Molecular diagnostic kits can be developed based on the proposed research. The kit can provide fast, accurate, sensitive, and quantitative diagnosis of leukemia specific chromosome translocations. Further more, a 96 well ELISA plate formated test could detect as many as 16 common translocations.