NIR FLUORESCENT LABELS FOR RNA SCREENING IN MICROARRAYS

DNA探针的灵敏荧光检测是应用所必需的 例如DNA测序、原位杂交和多组分阵列 用于检测mRNA表达。这样的应用通常限于 通过组织和细胞的自发荧光和其他 环境组成部分。因此，持续需要高度 生物研究中的高效、低背景荧光染料， 临床诊断学第一阶段的目的是确定 使用新型远红外和近红外（NIR）荧光标记的核酸 酸来检测mRNA与基因微阵列的杂交。我们有 选择酵母基因及其同源mRNA作为合适的测试系统， 在研究中也有广泛的应用。 在第二阶段，我们将合成一个家庭的荧光染料是一个优化 它们与DNA和RNA探针一起使用。我们还将开发红外扫描仪， 我们提供的标记探针可以有效地用于该领域。在 第二阶段标记探针的标记条件和使用参数 将精细化市场用于基因表达分析。 拟定商业应用： 这项技术的近期应用是在研究市场。 可直接用于标记的新型活性近红外染料 核酸可用于荧光原位杂交（FISH）， 在微阵列中检测mRNA表达并潜在地标记DNA 测序引物。

Sensitive fluorescent detection of DNA probes is required for applications such as DNA sequencing, in situ hybridization and multi-component arrays for detecting mRNA expression. Such applications are typically limited in sensitivity by autofluorescence of tissues and cells and other environmental components. Thus, there is a continuing need for highly efficient, low background fluorescent dyes in biological research and clinical diagnostics. Phase I is designed to determine the feasibility of using novel far-red and near-infrared (NIR) fluorescent labeled nucleic acids to detect the hybridization of mRNA to gene microarrays. We have chosen yeast genes and their cognate mRNAs as a suitable test system which may also have broad application in research. In Phase II we will synthesize a family of fluorescent dyes is an optimize their use with DNA and RNA probes. We will also develop the IR scanner so that the labeled probes we offer can be effectively used in the field. In Phase II the labeling conditions and use parameters of the labeled probes will be refined market for analysis of gene expression. PROPOSED COMMERCIAL APPLICATION: The near term applications of this technology are in the research market. Novel, reactive near-infrared dyes which can be directly employed to label nucleic acids can be used in fluorescence in situ hybridization (FISH), detecting mRNA expression in microarrays and potentially in labeling DNA sequencing primers.