ADF--A NOVEL CYTOKINE INVOLVED IN ADIPOGENESIS

肥胖是糖尿病(2型；NIDDM)的主要风险因素，但 脂肪细胞团是如何扰乱新陈代谢并产生胰岛素的 抵抗力、高血糖、高脂血症和高血压 糖尿病的特征尚不清楚。脂肪细胞分泌这两种糖尿病 促进分子(脂肪酸；肿瘤坏死因子-α)和至少一个可能 抗糖尿病(瘦素)。我们已经部分纯化了一种新的蛋白质 (细胞因子)，它也由成熟的脂肪细胞分泌，脂肪细胞 分化因子(ADF)。我们推测ADF也可能有 降血糖作用，因为，就像降血糖的噻唑烷二酮 药物，它诱导脂肪细胞分化，是一种正向调节因子 关于参与控制脂肪细胞增殖和 葡萄糖代谢。 为了研究ADF在肥胖和糖尿病中的作用，我们将使用 3T3-L1脂肪细胞条件培养液首次纯化ADF蛋白 同质性。在获得部分氨基酸序列后，我们将制作 抗体，克隆其基因，产生重组ADF蛋白。这些 研究将提供对脂肪细胞转化的基本了解 并提供一种蛋白质，可以导致 用于诊断测试和开发的重要试剂 肥胖、糖尿病及其相关疾病的治疗。 建议的商业应用： 由于ADF导致前脂肪细胞分化，该项目将 导致研究脂肪生成的重要试剂的开发和新颖 治疗学及其在脂肪组织形成和组织中的应用 工程学。

Obesity is a major risk factor for diabetes mellitus (type 2; NIDDM), but how the fat cell mass disturbs metabolism and produces the insulin resistance, hyperglycemia, hyperlipidemia, and hypertension that are the hallmarks of diabetes is unknown. Fat cells secrete both diabetes promoting molecules (fatty acids; TNF-alpha) and at least one that may be antidiabetic (leptin). We have partially purified a new protein (cytokine) which is also secreted by mature adipocytes, adipocyte differentiation factor (ADF). We hypothesize that ADF may also have antidiabetic action, since, like the antidiabetic thiazolidinedione drugs, it induces fat cell differentiation and is a positive regulator for genes involved in the control of adipose cell proliferation and glucose metabolism. In order to study the role of ADF in obesity and diabetes, we will using 3T3-L1 adipocyte conditioned medium to first purify the ADF protein to homogeneity. After obtaining partial amino acid sequences; we will make antibodies, clone its cDNA, and produce recombinant ADF protein. These studies will provide a basic understanding of adipocyte cell conversion and make available a protein that can lead to the development of important reagents for use in the development of diagnostic tests and treatments of obesity, diabetes and their associated diseases. PROPOSED COMMERCIAL APPLICATIONS: Since ADF causes differentiation of preadipocyte cells, this project will lead to development of important reagents to study adipogenesis and novel therapeutics with applications in adipose tissue formation and tissue engineering.