PRO FLUORESCENT NUCLEOTIDE ANALOGS FOR GENETIC ANALYSIS

描述：(改编自申请人的摘要)核苷酸类似物 标记有可检测的部分，如荧光染料，被广泛使用 用于研究和诊断分析。链末端核苷酸 类似物(双脱氧核苷酸)在DNA测序中特别有用 应用和引物延伸分析，如微测序或 遗传位分析(GBA)。他们提议开发一套独特的 亲荧光双脱氧核苷酸类似物将使更快 和敏感的突变检测也为 聚合酶活性分析，例如细胞存活率。建议数 然而，类似物作为游离核苷酸是非荧光的。 通过DNA聚合酶结合到DNA中，释放出一个荧光团 变成了溶液。释放到溶液中的荧光量为 与聚合酶活性直接相关，从而提供了实时 特定碱基类似物的掺入试验。 他们提议合成并测试亲荧光的概念 在本赠款的第一阶段期间使用双脱氧核苷酸类似物 成功，开发所有四个碱基类似物和分析条件 第二阶段。该项目的成功完成将导致一系列 具有广泛用途的亲荧光核苷酸类似物在 遗传和生化研究及诊断。 建议的商业应用：不可用

DESCRIPTION: (adapted from applicant's abstract) Nucleotide analogs labeled with detectable moieties, such as fluorescent dyes, are widely used in research and diagnostic assays. Chain terminating nucleotide analogs (dideoxynucleotides) are especially useful in DNA sequencing applications and primer extension assays, such as minisequencing or genetic bit analysis (GBA). They propose to develop a unique set of pro-fluorescent dideoxynucleotide analogs that will enable more rapid and sensitive mutation detection as well a provide unique substrates for polymerase activity assays, such as for cell viability. The proposed analogs are non-fluorescent as free nucleotide, however, upon incorporation into DNA by a DNA polymerase, a fluorophore is released into solution. The amount of fluorescence released into solution is directly correlated with polymerase activity, thus providing a real-time assay for incorporation of a specific base analog. They propose to synthesize and test the concept of pro-fluorescent dideoxynucleotide analogs during Phase I of this grant and if successful, develop all four base analogs and assay conditions during Phase II. Successful completion of this project will result in a series of pro-fluorescent nucleotide analogs with broad ranging utility in genetic and biochemical research and diagnostics. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE