ENZYME THERMISTORS AS GLUCOSE SENSORS

描述(改编自申请人的摘要)：本提案旨在 酶包被热敏电阻用作葡萄糖的可行性研究 I型糖尿病的感应器。申请者和他的团队之前 在这种传感器的开发上做了大量的工作 尽管是出于不同的目的，他们发现热敏电阻有一个数字 提出了比电极式传感器更具优势的推广方案。 总体目标是共价固定化葡萄糖的结合。 在合适的涂层的微薄的一层上添加氧化物酶和过氧化氢酶 已应用于热敏电阻的玻璃体。几个可能的联合- 目前正在研究聚合物作为涂层的稳定性。 从诺氏拟青霉中分离出葡萄糖氧化酶，据报道该酶具有 极低公里的氧气，将被用来试图克服“氧气” 使用来自黑曲霉的酶所遇到的“缺陷”问题 该酶被成功地固定在热敏电阻上，动力学和 传感器的物理特性将在体外进行研究。然后是一个 酶-热敏电阻的体内稳定性研究将于#年进行。 兔，皮下和静脉内测量对 血糖水平的突然变化。该电子仪器和 处理来自热敏电阻的信号所需的计算机程序具有 已经在美国国立卫生研究院设计了一个原型 本项目将使用P.I.S实验室的仪器。

DESCRIPTION (Adapted from applicant's abstract): This proposal aims to do a feasibility study on the use of enzyme coated thermistors as glucose sensors to in type I diabetes. The applicant and his group have previously done a considerable amount of work on the development of such sensors albeit for a different purpose and they find the thermistor has a number of advantages over the electrode-type sensors presenting being promoted. The overall aim is to covalently immobilize a combination of glucose oxidase and catalase onto a micro-thin layer of a suitable coating that has been applied to the glass body of the thermistor. Several possible co- polymers are presently being investigated for stability as a coating. Glucose oxidase isolated from P. notatum, which has been reported to have a very low Km for Oxygen, will be use to try to overcome the "oxygen deficit" problems encountered with use of the enzyme from A. niger. After the enzyme is successfully immobilized on the thermistor, the kinetic and physical properties of the sensors will be investigated in vitro. Then a stability study of the enzyme-thermistors in vivo will be conducted in rabbits, both subcutaneously and intravenously to measure response time to sudden changes in blood glucose levels. The electronic instrument and the computer program needed to process the signals from the thermistors have already been designed at the National Institutes of Health, an a prototype instrument in the P.I.'s laboratory will be used in this projects.