RAMAN AND TIME RESOLVED RAMAN STUDIES OF HEME PROTEINS

描述：该项目涉及到亚铁血红素的总体长期目标 蛋白质的目的是在分子水平上理解 相关的多肽与共同的血红素基团相互作用，产生 这类蛋白质具有显著的功能多样性。 基本的策略是应用强大的光谱探测器，如 共振拉曼光谱和时间分辨共振拉曼光谱 系统地操纵蛋白质，以揭示结构和 调节血红素反应性的动态相互作用。最近开发的 方法论现在允许几个根本上重要的问题 走近了。例如，分子振动的同位素灵敏度是 被利用来选择性地探测正常运行的本机中的单个站点 并在合理的位点特异性突变体中揭示出更好的 对变构协作性的理解。此外，小说的应用 快速混合技术，加上可用的定点突变体，将 促进彻底调查活跃的站点结构 以前无法接触到的重要氧化血红素的催化中间体 细胞色素P450等酶和各种过氧化物酶。

DESCRIPTION: The general long-term goal of this program involving heme proteins is to attain a molecular understanding of the mechanisms by which the associated polypeptides interact with a common heme group to produce the remarkable functional diversity which characterizes this class of proteins. The essential strategy is to apply powerful spectroscopic probes such as resonance Raman and time-resolved resonance Raman to the native and systematically manipulated proteins in order to reveal the structural and dynamic interactions which regulate heme reactivity. Recently developed methodology now allows several fundamentally important questions to be approached. For example, isotope sensitivity of molecular vibrations is exploited to selectively probe individual sites in functioning native hemoglobin and in rational site specific mutants to reveal a better understanding of allosteric cooperativity. Also, application of novel rapid-mixing techniques, coupled with available site-specific mutants will facilitate a thorough investigation of the active site structure of previously inaccessible catalytic intermediates of important oxidative heme enzymes such as cytochrome P450 and various peroxidases.