A novel biocatalyst platform for biobased-synthesis of 1,3-propanediol

生物制造已成为绿色、可再生、复杂的生物燃料、药物和精细化学品合成的化学催化的一种有前途的替代方案。与体内发酵方法相比，无细胞化学生物合成具有额外的优势，因为它使单独产生的酶能够即插即用地组装成最佳的级联反应，多样化的反应条件，并直接进入反应环境。FabricNano开发了一种合成生物学，基于DNA的纳米反应器平台技术，有可能改变英国的化学合成行业。他们的方法极大地改善了多酶级联动力学的反应动力学，以快速获得产品，并有助于化学生产的绿色和可持续发展。FabricNano已经确定了一种多酶级联反应，将废甘油转化为1，3-丙二醇(PDO)。生物乙醇/生物柴油生产产生甘油废物，价值20便士/公斤；PDO的价值是GB 9/kg，因为它可用于许多应用(塑料、化妆品、个人护理、清洁产品)。对PDO的需求很大，但只有四家领先的制造商的年产量约为142.5千吨/年。PDO化学处理技术是能源密集型的，消耗金属催化剂，并创造危险的工作条件。最先进的方法通过发酵将糖和多元醇转化为PDO。然而，发酵依赖于漫长的酵母培养，并且消耗大量的能源和淡水。由于Covid危机限制了英国生物柴油/生物乙醇生产商的市场(休闲旅行和货运服务的减少降低了需求)，FabricNano已经找到了一个机会：1.通过为低价值废物创造附加值，帮助生物乙醇/生物柴油生产商在Covid危机期间保持盈利/生存能力。创建国内PDO供应链(目前英国没有生产商)。该项目将有助于展示FabricNano生物催化剂在工业规模上的可行性。它还将证明FabricNano技术用于耦合级联酶反应，将低价值的可再生原料转化为高价值的产品，这代表了可再生绿色化学的关键。

Biomanufacturing has emerged as a promising alternative to chemocatalysis for green, renewable, complex synthesis of biofuels, medicines, and fine chemicals. Cell-free chemical biosynthesis offers additional advantages over in vivo fermentation methods, by enabling plug-and-play assembly of separately produced enzymes into an optimal cascade, versatile reaction conditions, and direct access to the reaction environment.FabricNano have developed a synthetic biology, DNA-based nanoreactor platform technology with the potential to transform the UK's chemical synthesis industry.Their approach vastly improves reaction kinetics of multi-enzyme cascade kinetics to yield product quickly, and can contribute to the greening and sustainability of chemical production.FabricNano have identified a multi-enzyme cascade that converts waste glycerine into 1,3-Propanediol (PDO).Bioethanol/biodiesel production creates waste glycerine, valued at 20p/kg; PDO is valued at £9/kg, due to it utility across many applications (plastics, cosmetics, personal care, cleaning products)There is significant demand for PDO, but just four leading manufacturers have an annual output c. 142.5 kilotons/year. PDO chemical processing techniques are energy intensive, consume metal catalysts, and create dangerous working conditions. State-of-the-art approaches convert sugars and polyols to PDO via fermentation. However, fermentation relies on lengthy yeast culture, and consumes significant energy and fresh water.Because the Covid crisis is restricting the market for the UK's biodiesel/bioethanol producers (less leisure travel and freight services has lowered demand), FabricNano have identified an opportunity to:1. Help bioethanol/biodiesel producers to remain profitable/viable operators during the Covid crisis by creating added-value for a low-value waste stream.2. Create a domestic supply chain for PDO (currently no producers in the UK).The project will help to demonstrate the viability of FabricNano's biocatalyst at an industrial scale. It will also prove utility of FabricNano technology for coupling cascading enzyme reactions for the conversion of low value renewable feedstocks into high value products, which represents a keystone of renewable green chemistry.