CELL-MATRIX INTERACTIONS IN AMPHIBIAN DEVELOPMENT

描述：本提案来自一位已成立的研究者， 在这一领域发表了出色的作品。 他试图继续 表征中胚层和细胞外 在早期非洲爪蟾胚胎中形成的基质。 当中胚层细胞在 胚胎，他们成为能动的，并通过一个复杂的和不了解的机制， 在胚胎中移动。 当他们这样做的时候，他们的行为会根据 它们是否会形成背侧、腹侧、前部或后部结构。 细胞外基质是侵入中胚层的基质之一 由囊胚腔顶的细胞分泌。 这是一种相互作用， 中胚层细胞和细胞外基质糖蛋白， 这个提议。 目的是：1）确定细胞基质的作用 在原肠胚形成过程中使用功能阻断剂的相互作用，2） 开发整合素激活位点的“报告者”， 监测受体活化的阶段和区域特异性变化，以及3） 看看中胚层基因表达的模式是否需要细胞-ECM相互作用。

DESCRIPTION: This proposal is from an established investigator who has published excellent work in this field. He seeks to continue to characterize the interactions between the mesoderm and the extracellular matrix as it forms in early Xenopus embryos. As mesoderm cells form in the embryo, they become motile, and by a complex and ill-understood mechanism, move inside the embryo. As they do so, they behave differently according to whether they will form dorsal, ventral, anterior or posterior structures. One of the substrata of the ingressing mesoderm is the extracellular matrix secreted by the cells of the blastocoel roof. It is the interaction between mesoderm cells and the extracellular matrix glycoprotein that is the subject of this proposal. The aims are 1) to establish the roles of cell-matrix interactions during gastrulation using function-blocking reagents, 2) To develop "reporters" of sites of integrin activation that will be used to monitor stage and region-specific changes in receptor activation, and 3) to see if patterns of mesodermal gene expression require cell-ecm interaction.