POROUS POLYMER RODS AS CHROMATOGRAPHIC OR REACTIVE MEDIA

本续展方案针对的是设计、准备和 测试整体式“模制”分离介质的全新形状和 补充当前基于珠粒的压缩介质的格式，并提供新的 以及无与伦比的研究、检测、隔离和 生物活性分子的分离。新的分离介质是 基于连续的大孔聚合物直接模制成 分离装置的外壳而不是由小珠制成 机械地填充到柱子中。“模塑”的媒体提供了许多 例如易于准备和易于处理的优点， 分离化学的重复性、多功能性、无与伦比的能力 结合了孔隙度和化学、紧实度和 简单的纵向扩展。 与悬架的两相系统特性形成对比 聚合用来制备珠状分离介质，模压成型 介质是从单一阶段制备的，允许使用许多功能 或者是不能用于常规珠子的水溶性单体。这个 易于准备是去除尺寸的另一个重要因素 分馏和包装步骤已知会极大影响 填料塔的效率。连续介质的制备方法为 简单、无浪费、劳动强度低的制备方法 珠子，导致每台设备的单位成本较低。的多功能性 分离化学进一步增强的能力是混合几个 化学或实现组成和/或孔隙度的梯度 单模介质。新型模压整体的其他优点包括 显著地强化了传质，因为所有的液体流动 通过连接到小孔网络的大毛孔 有很大的表面积。这允许极快地分离 生物聚合物，并导致动力学的显著改善 固定化生物催化剂。 总体而言，我们的目标是显著提高分析、 可用于处理生物的准备和诊断工具 体液。我们开发的新媒体将为这两个领域做出更好的贡献 高性能液体等领域的性能和新功能 层析、电色谱、毛细管和自由流区 电泳学、诊断学、检测学、酶固定化等。这个 模压系统的无与伦比的多功能性及其独特的功能使 它们非常适合从最小的毛细管到各种产品 格式到用于分离药物、多肽、 蛋白质，以及寡聚或多核苷酸。

This continuation proposal is aimed at the design, preparation, and testing of monolithic "molded" separation media in entirely new shapes and formats that complement current bead-based packed media and provide new and unmatched capabilities for the study, detection, isolation, and separation of biologically active molecules. The new separation media are based on a continuous body of macroporous polymer molded directly into the housing of the separation device rather than made of small beads mechanically packed into the column. The "molded" media offer many advantages such as ease of preparation and ease of handling, reproducibility, versatility of separation chemistry, unmatched ability to incorporate gradients of porosity and of chemistry, compactness, and simple scale-up. In contrast to the two-phase systems characteristic of the suspension polymerization used to prepare bead-shaped separation media, the molded media are prepared from a single phase allowing the use of many functional or water-soluble monomers that cannot be used for conventional beads. The ease of preparation is another important factor that removes the size fractionation and packing steps which are known to greatly affect the efficiency of packed columns. The preparation of the continuous media is simple, waste-free, and less labor-intensive that the preparation of beads, leading to a low unit cost per device. The versatility of separation chemistry is further enhanced by the ability to blend several chemistries or achieve gradients of composition and/or porosity in a single molded medium. Other advantages of the new molded monoliths include remarkably enhanced mass transfer because all of the liquid phase flows through their large pores that are connected to a network of small pores with a large surface area. This allows extremely fast separations of biopolymers and leads to a dramatic improvement in kinetics for immobilized biocatalysts. Overall, our targets are to significantly enhance the array of analytical, preparative, and diagnostics tools available for handling biological fluids. The new media we develop will contribute to both better performance and new capabilities in areas such as high-performance liquid chromatography, electrochromatography, capillary and free-flow zone electrophoresis, diagnostics, detection, enzyme immobilization, etc.. The unequaled versatility of the molded systems and their unique features make them ideal for a broad range of products from the smallest capillary format to large preparative systems for the separation of drugs, peptides, proteins, and oligo- or polynucleotides.