CORE--ANIMAL EXPERIMENTATION RESOURCE

描述：(申请人描述)对生物样本中存在的各种自分泌、旁分泌和神经分泌因子的影响的生物和临床研究需要准确和可靠地测量这些产品的能力。同样，对药物作用的评估需要测量这些药物及其在组织和液体提取物中的代谢物，以允许定义动力学。这些具体目标构成了分析实验室主要职能的理论基础。这一设施自1989年以来一直存在。这是一间600平方英尺的套房。英国《金融时报》化学实验室，200平方米。英国《金融时报》4摄氏度冷藏室，2个设备室，其中包括-70摄氏度和-30摄氏度的冰柜，旋转真空/冷冻干燥器，以及伽马和闪烁计数器。该单元完全能够偶联抗原，产生多克隆兔抗体，偶联碘化配体，抗体的表征和检测。此外，它还拥有专门的HPLC，用于鉴定柱状免疫反应性和药物的气相色谱。该实验室拥有各种多肽(脑啡肽、神经肽、速激肽、甘丙肽、蛙黄素、降钙素基因相关肽)、胺和代谢物(儿茶酚胺和吲哚胺)、氨基酸、嘌呤(腺苷、ATP/ADP/AMP)、前列腺素(PGE1/2；LTB4/C4/D4)和细胞因子(TNF、IL1)的在线分析。除了这些激素，各种药物及其代谢物也要进行分析。这些药物包括鸦片类药物(吗啡、度冷丁、芬太尼及其衍生物、美沙酮、可待因)、局麻药(利多卡因、布比卡因、美西律)、注射麻醉剂(异丙酚、咪达唑仑、巴比妥酸盐)和乙醇。该设施配备了完整的数据管理设备，配备了Windows NT工作站和(安全的)校园范围内启用E以太网的结果分发。

Description: (Applicant's Description) Biological and clinical research on the effects of various autocrine, paracrine and neurocrine factors present in biological samples require the ability to accurately and reliably measure these products. Similarly, the assessment of drug action necessitates the measurement of these agents and their metabolites in tissue and fluid extracts to permit definition of kinetics. These specific aims constitute the rationale for the primary function of the Analytical Laboratory. This facility has been in existence since 1989. It is a suite consisting of a 600 sq. ft. chemistry laboratory, a 200 sq. ft. 4 degree C cold room, 2 equipment rooms, which contain -70 and -30 degree C freezers, spin vac/lyophilizer, and gamma and scintillation counters. The unit is fully capable of coupling antigens, creating polyclonal rabbit antibodies, coupling of the iodinated ligand, characterization of the antibody and the assay. In addition, it has dedicated HPLCs for identification of column immunoreactivity and gas chromatography for drugs. The laboratory has on-line assays for a variety of peptides (enkephalins, NPY, tachykinins, galanin, bombesin, CGRP), amines and metabolites (catecholamines and indolamines), amino acids, purines (adenosine, ATP/ADP/AMP), prostanoids (PGE1/2; LTB4/C4/D4), and cytokines (TNF, IL1). In addition to these hormones, a variety of drugs and their metabolites are also subject to analysis. These include opiates (morphine, meperidine, fentanyl and derivatives, methadone, codeine), local anesthetics (lidocaine, bupivacaine, mexiletine) injectable anesthetic (propofol, midazolam, barbiturates) and ethanol. The facility is fully equipped for data management with a Windows NT workstation and (secured) campus-wide E thernet-enabled results distribution.