CORE--HYBRIDOMA LIBRARY / MONOCLONAL ANTIBODY PRODUCTION

杂交瘤文库/单克隆抗体生产共享资源（杂交瘤共享资源）为NCCC研究者提供质量受控的单克隆抗体（mAb），作为从ATCC或个体研究者获得的杂交瘤细胞系的纯化蛋白和/或上清液。该设施保存了来自100多个细胞系的上清液和单克隆抗体的表征和质量控制库存。对于那些只需要一个给定抗体的小样本的研究来说，它特别具有成本效益，否则将导致每个抗体收取250至500美元的个人赠款。该资源还建议研究人员创建新的杂交瘤以及根据需要生产和纯化大量抗体。在过去的一年中，该资源仅由NCCC调查人员使用;这些抗体的供应没有收费系统，这些抗体通常来自专利细胞系。在过去的一年里，这种共享资源估计比可比抗体的商业成本节省了41，000至100，000美元。

The Hybridoma Library/Monoclonal Antibody Production Shared Resource (Hybridoma Shared Resource) provides NCCC investigators with quality-controlled monoclonal antibodies (mAbs) as purified proteins an d/or supernatants from hybridoma cell lines that have been obtained from the ATCC or from individual investigators. This facility maintains characterized and quality-controlled stocks of supematants and monoclonal antibodies from more than one hundred cell lines. It is especially cost- effective for studies that require only a small sample of a given antibody and would otherwise result in a charge to individual grants of $250 to $500 per antibody. This Resource also advises investigators on both the creation of new hybridomas and the production and purification of large quantities of antibodies as needed. During the past year this Resource was used exclusively by NCCC investigators; there is no charge-back system for supply of these antibodies, which often come from patented cell lines. Over the past year, this Shared Resource is estimated to have achieved a net savings of between $41,000 and $100,000 over the commercial cost of comparable antibodies.