ANALYSIS OF THE HDLG-DEPENDENT PROTEIN COMPLEX

描述：根据他们的工作和其他实验室的工作， 研究人员认为hDlg是一种成分， 成核剂，质膜的多蛋白质复合物， 相关的信号分子。 这个假设是基于 观察到hDlg序列与APC、Abl、Crk、Lck的结合， 称为PBK1（PDZ结合激酶1）的双特异性蛋白激酶， T/SXV基序通道蛋白，含SH3蛋白，聚脯氨酸- 含有蛋白质和/或蛋白质4.1oezrin。 拟议研究 我将通过以下方法来检验这一假设：（1）完善互动地图 hDlg和4.1/ERM蛋白之间的结构域以及这种结构域的作用 靶向膜的相互作用;（2）定量和细化 对hDlg与其自身的关联的理解;（3）定量和 完善合作伙伴;（4）评估PDZ的影响- 介导的关联hDlg和推定的激酶鉴定， 酵母双杂交筛选。 长期目标是更好地理解 这种肿瘤的作用方式和生化特征 抑制蛋白

DESCRIPTION: Based on their work and that of other laboratories, these investigators propose that hDlg is a component, possibly the essential nucleating agent, of a multi-protein complex of plasma membrane- associated signaling molecules. This hypothesis is based on the observed binding of hDlg sequences to APC, Abl, Crk, Lck, a new putative dual-specificity protein kinase called PBK1 (PDZ-Binding Kinase 1), T/SXV motif channel proteins, SH3-containing proteins, polyproline- containing proteins, and/or protein 4.1 oezrin. The proposed research will test this hypothesis by: (1) refining the map of interaction domains between hDlg and 4.1/ERM proteins and the role of this interaction in targeting to the membrane; (2) quantifying and refining the understanding of hDlg association with itself; (3) quantifying and refining the partners; and (4) assessing the implication of the PDZ- mediated association between hDlg and a putative kinase identified by a yeast two-hybrid screen. The long-term goal is to understand better the mode of action and biochemical characteristics of this tumor suppressor protein.