ANESTHETIC ACTIONS ON INHIBITORY GABAERGIC INTERNEURONS

描述：(改编自申请者的摘要) 研究人员的目的是了解全身麻醉的作用机制。 麻醉期间神经元兴奋性减弱的部分原因是 增强GABA介导的突触抑制。这个主要的方向是 建议研究麻醉对抑制GABA能的作用 中间神经元。中间神经元通过释放GABA来调节突触抑制。 全细胞记录和红外成像现在使研究成为可能 麻醉作用于以往难以接触到的中间神经元。 出乎意料的是，因为全身麻醉药通常会抑制递质 释放，是初步发现全身麻醉药增加了 抑制性中间神经元自发释放GABA。增强型GABA 释放能与麻醉剂-突触后GABA受体的作用相结合 加强抑制。拟议的研究将确定 麻醉药对抑制性GABA能中间神经元的影响 这些行动背后的机制，并决定这些行动的后果 对神经元兴奋性的影响。调查人员认为，这些 对抑制性GABA能中间神经元的研究将提供信息 关于麻醉作用的机制。

DESCRIPTION: (Adapted from the applicant's abstract) The goal of the investigators is to understand mechanisms of general anesthetic action. Attenuated neuronal excitability during anesthesia results in part from enhanced GABA-mediated synaptic inhibition. The major direction of this proposal is to investigate anesthetic actions on inhibitory GABAergic interneurons. Interneurons mediate synaptic inhibition by releasing GABA. Whole-cell recordings and infrared imaging make it now possible to study anesthetic actions on previous difficult to access interneurons. Unexpectedly, because general anesthetics usually depress transmitter release, is the preliminary finding that general anesthetics increase the spontaneous release of GABA from inhibitory interneurons. Enhanced GABA release can combine with anesthetic-postsynaptic GABA receptor actions to augment inhibition. The proposed study will identify the effects of anesthetics on inhibitory GABAergic interneurons, characterize the mechanisms underlying these actions, and determine the consequences of these effects on neuronal excitability. The investigators believe that these studies on inhibitory GABAergic interneurons will provide information regarding mechanisms of anesthetic action.