GENETIC BIOMARKERS WITH CALIFORNIA SEA LIONS

人口聚集地附近海洋环境的污染可能暴露 海洋哺乳动物对致癌和致突变化学物质的敏感性。人类居住在 这些区域附近可能共享一些相同的食物来源，使它们暴露在 类似的风险。我们建议采用一种既定的监测方法 人类人群暴露诱变剂，放射自显影HPRT突变体 淋巴细胞测定，用于加利福尼亚海狮(Zalophus) Calfornianus)。检测条件和基线突变频率将为 用从海上圈养人口中获得的血液样本建立 世界，德克萨斯州圣安东尼奥作为“阳性”对照，来自这一区域的淋巴细胞 人群将暴露在活性多芳烃中，例如 苯并(A)芘二醇-环氧化物的体外药敏试验 一种特有的环境诱变剂。人类淋巴细胞也将 在体外进行治疗以作比较。从15-20名自由滞留的人身上采集的血样 活海狮将从#年的海洋哺乳动物中心获得 加利福尼亚州索萨利托将对淋巴细胞进行HPRT突变频率的检测。 多环芳烃的组织水平数据和 由海洋哺乳动物中心测定的有机氯水平将是 用来分析突变频率与暴露的关系。

Pollution of marine environments near human population centers can expose marine mammals to carcinogenic and mutagenic chemicals. Humans residing near these areas may share some of the same food sources, exposing them to similar risks. We propose to adapt an established method for monitoring human populations for mutagen exposure, the autoradiographic hprt mutant lymphocyte assay, for use with California sea lions (Zalophus californianus). Assay conditions and baseline mutant frequencies will be established with blood samples obtained from a captive population at Sea World, San Antonio, Tx. As a "positive" control, lymphocytes from this population will be exposed to a reactive polyaromatic hydrocarbon, such as a benz(a)pyrene diol - epoxide, in vitro to determine their sensitivity to a characteristic environmental mutagen. Human lymphocytes will also be treated in vitro for comparison. Blood samples from 15-20 stranded free- living sea lions will be obtained from the Marine Mammal Center in Sausalito, CA. Lymphocytes will be assayed for frequencies of hprt mutants. Data on tissue levels of polycyclic aromatic hydrocarbons and organochlorine levels, as determined by the Marine Mammal Center, will be used to analyze the relationship between mutant frequency and exposure.