CONSTRUCTION OF A NATIONAL RESOURCE FOR ZEBRAFISH

在过去的几年里，我们对 脊椎动物的发展，主要是基于一些模型遗传学的工作， 有机体斑马鱼是这些模式生物中最新的。因为 身体模式的基本原理在 所有脊椎动物的胚胎发生，从胚胎发育研究中获得的见解 斑马鱼将对人类健康和疾病产生影响。此外，委员会认为， 对这种生物体的研究符合《动物福利法》的意图 因为许多高等脊椎动物的使用现在可以被这种 低等脊椎动物 对斑马鱼的系统遗传研究始于俄勒冈州大学 多年来尤金是唯一一个演出的地方。最近， 然而，国际上对这一生物体的兴趣大大增加; 斑马鱼的胚胎学和遗传学研究以及最近的 超过7,000个基因突变的鉴定导致了戏剧性的 使用这种微生物研究 脊椎动物发育的基本机制。目前，这些基因库 分布在28个国家的100多个实验室。使 为新的突变体提供空间，实验室必须停止他们目前的一些实验。 其中许多已经永久消失。斑马鱼研究 社区迫切需要一个集中的网站来保存和跟踪 这些股票，并促进其分配给研究人员，从而 支持和促进研究机会 重复劳动。 我们建议建立一个设施，以维持野生型和突变体股票 并使这些种群广泛提供给 国际研究界。需要一个库存中心，因为它可以 取消各实验室维持库存的要求 他们无法研究，它可以提供动物以较低的成本， 最重要的是，它可以确保更高的 可能的质量和一致性水平。具体而言，我们建议： 1.建立一个库存中心，作为 材料和信息。 2.向研究界广泛提供信息。 3.开发改善健康的方法。

The last several years have witnessed an explosion in our understanding of vertebrate development, largely based on work from a few model genetic organisms. The zebrafish is the newest of these model organisms. Because the basic principles of body patterning appear similarly during embryogenesis of all vertebrates, insights gained from work on embryonic zebrafish will have implications for human health and disease. Moreover, research on this organism meets the intent of the Animal Welfare Act because use of many higher vertebrates can now be replaced by use of this lower vertebrate. Systematic genetic research on zebrafish began at the University of Oregon and for many years Eugene was the only place it was performed. Recently, however, international interest in this organism has grown tremendously; studies of the embryology and genetics of zebrafish and the recent identification of over 7,000 genetic mutations have led to a dramatic increased in the number of laboratories using this organism to study the basic mechanisms of vertebrate development. Currently these genetic stocks are distributed among more than 100 laboratories in 28 countries. To make room for new mutants, laboratories must discontinue some of their current stocks many of which are permanently lost. The zebrafish research community desperately needs a centralized site to preserve and keep track of these stocks and facilitate their distribution to researchers, thus supporting and promoting research opportunities whole preventing duplication of effort. We propose to construct a facility to maintain wild-type and mutant stocks of zebrafish and to make these stocks widely available to the international research community. A stock center is needed because it can eliminate the requirement of individual laboratories to maintain stocks they are unable to study, it can provide animals at lower cost than individual laboratories, and most importantly, it can ensure the higher possible levels of quality and uniformity. Specifically we propose: 1. To establish a stock center to serve as a central repository for materials and information. 2. To make information widely available to the research community. 3. To develop methods for improving health.