STRUCTURAL ORGANIZATION OF THE MAMMALIAN GOLGI COMPLEX

高尔基复合体是糖脂生物合成、精加工 分泌性血浆的糖蛋白加工和分选步骤 膜和溶酶体/液泡蛋白。 在哺乳动物细胞中， 由一个紧密的双核复合体组成， 高尔基体膜的浓度，高尔基体堆叠，通过 管状延伸。 这种非核定位是 与细胞器相关的运动蛋白，可能是动力蛋白的成员 家庭，和微管方向相对于微管 组织中心（MTOC）。 在高尔基体中，细胞器 蛋白质以极化的方式组织，如通过形态学所辨别的， 居住和功能。 在本申请中，我们提出使用 一种多标记的方法来探测脑池堆栈如何 是紧密相连的 这种多标记方法只有在 通过产生转染表位的稳定细胞系 标记的高尔基体糖基转移酶。 尼尔森博士一直是 这个

The Golgi complex is the site of glycolipid biosynthesis, finishing steps in glycoprotein processing and sorting of secretory, plasma membrane and lysosomal/vacuolar proteins. In mammalian cells, it consists of a compact, juxtanuclear complex in which individual concentrations of Golgi membranes, Golgi stacks, are interconnected by tubular extensions. This juxtanuclear localization is a consequence of organelle associated motor proteins, presumably members of the dynein family, and microtubule orientation with respect to the microtubule organizing center (MTOC). Within a Golgi stack, the organelle is organized in a polarized fashion as discerned by morphology, protein residence and function. In the present application, we propose to use a multimarker approach to probe key aspects of how the cisternal stack is held together. Such a multimarker approach has only become possible through the generation of stable cell lines transfected for epitope tagged Golgi glycosyltransferases. Dr. Nilsson has been a pioneer in this.