ESTROGEN RECEPTOR FUNCTION AND THE NUCLEAR MATRIX

人们越来越意识到，核区划是一种 核代谢的基本成分。这项建议的目的是 研究雌激素受体作为一个模型来理解细胞核是如何 比较作用会影响转录活性。要做到这一点 Goal，内质网在不同激素下靶向的亚核区域 将使用高分辨率显微镜和 与其他特定亚核标记的共定位研究 域名。雌激素受体内负责 与核基质的相互作用将由 缺失构建体和嵌合蛋白的生成。最后， 内质网与转录共激活因子的相互作用 回归分析将在核骨骼的背景下进行，以确定 这些因子的亚核区隔化如何调控基因 抄写。

There is a growing awareness that nuclear compartmentation is an essential component of nuclear metabolism. This proposal is aimed at studying the estrogen receptor as a model to understand how nuclear comparmentation affects transcriptional activity. To achieve this goal, the subnuclear domains targeted by ER under varying hormonal conditions will studied using high resolution microscopy and colocalization studies with other markers of specific subnuclear domains. The regions within the estrogen receptor responsible for interaction with the nuclear matrix will be analyzed by the generation of deletion constructs and chimeric proteins. Finally, the interaction of ER with transcriptional coactivators and regressors will be analyzed in a nucleoskeletal context to determine how subnuclear compartmentation of these factors regulates gene transcription.