IRELAND SCIENTIFIC PROGRAM

NCDDG的这个子计划的目标是发现自然 海洋无脊椎动物生产的产品和/或相关产品 氰胺类肿瘤药物中显示活性的微生物 屏幕(CODS)。为实现这一目标而制定的具体目标包括 如下所述。 一、收集各种海洋无脊椎动物(340种 每年)，来自不同的地理位置和生态位。 大部分样本(约300个)将从以下地点获得 菲律宾作为与埃德加多·戈麦斯博士合作的一部分， UP-MSI主任。样本也将从浅水中提取。 百慕大的珊瑚礁、公海上层海洋群落和底栖海洋群落。 2.从海洋栖息地分离和培养微生物 在莱德勒实验室。显示活动的生物体将是 重新发酵，供分离研究之用。 3.进行生物测定指导下的有效成分的分离 上述生物的提取物，并提供这些 用于生物评价的代谢物。 4.对上述活性成分进行结构表征 并确定其作用机制(S) 尽最大可能。 5.对不同物种的生物多样性进行系统清查 菲律宾海洋生态系统内的栖息地。这些研究将 对妥善管理和保护菲律宾非常重要 海洋环境。

The objective of this sub-program of the NCDDG is to discover natural products roduced by marine invertebrate animals and/or associated micro-organisms that display activity in Cyanamid's Oncology Drug Screen (CODS). The specific aims developed to accomplish this goal are described below. I. To collect a variety of marine invertebrate animals (340 species annually) from different geographical locales and ecological niches. The majority of the samples (approximately 300) will be obtained from the Philippines as part of a collaboration with Dr. Edgardo Gomez, Director, UP-MSI. Samples will also be obtained from the shallow water reef, open ocean pelagic and benthic marine communities of Bermuda. 2. Micro-organisms from marine habitats will be isolated and cultured at Lederle Laboratories. The organisms that display activity will be re-fermented and supplies for isolation studies. 3. To perform bioassay guided isolation of active constituents from the extracts of the above mentioned organisms and to provide these metabolites for biological evaluation. 4. To structurally characterize the active constituents from the above mentioned organisms and to determine their mechanism(s) of action to the extent possible. 5. To perform systematic inventories of biodiversity of the various habitats within the Philippine marine ecosystem. These studies will be important for proper management and conservation of the Philippine marine environment.