Defining New Functions in DNA Replication and DNA Damage Response Genes

定义 DNA 复制和 DNA 损伤反应基因的新功能

基本信息

项目摘要

Scientific goals: In the budding yeast S. cerevisiae, genome-wide collections of mutant strains have been employed extensively to elucidate biological function on a large scale. The resulting information has been used to place genes in pathways, to identify points of intersection between different pathways and to assign gene function to novel proteins. One flaw of these studies stems from the nature of the mutant reagents in these genome-wide collections, where gene function has been inactivated by either deletion or conditional depletion. Since many, if not all, proteins execute more than one function, such mutations are potentially pleiotropic. A potential solution is to employ separation-of-function mutations that eliminate a single biological function of a protein. However, identification of this particular class of alleles in the past has been a logistic hurdle even for single genes. This research project is based on a newly developed strategy for large-scale isolation of separation-of-function alleles which will be applied to a set of inter-related genes involved in DNA replication and response to DNA damage. Since very few mutations have been isolated for many of these genes, this research should define new functions in the DNA replication and DNA damage response pathways. Broader impacts: The scientific goals of this research project are closely intertwined with two broader impacts. First, the resulting mutations will be made widely available to the yeast community, with a particular focus on incorporation of these new reagents into genome-wide systems analysis. Genetic networks constructed from separation-of-function missense mutations are likely to uncover previously unappreciated interfaces that were missed in prior systems analysis which employed currently available mutant strains. Second, this project will rely heavily on entry-level researchers (undergraduate and high school students) who will be responsible for generating the panels of separation-of-function mutations. This experience will allow very junior researchers to play a central role in a significant research project and also introduce them to the critical function that mentoring plays in the biomedical research community.
Scientific goals: In the budding yeast S. cerevisiae, genome-wide collections of mutant strains have been employed extensively to elucidate biological function on a large scale. The resulting information has been used to place genes in pathways, to identify points of intersection between different pathways and to assign gene function to novel proteins. One flaw of these studies stems from the nature of the mutant reagents in these genome-wide collections, where gene function has been inactivated by either deletion or conditional depletion. Since many, if not all, proteins execute more than one function, such mutations are potentially pleiotropic. A potential solution is to employ separation-of-function mutations that eliminate a single biological function of a protein. However, identification of this particular class of alleles in the past has been a logistic hurdle even for single genes. This research project is based on a newly developed strategy for large-scale isolation of separation-of-function alleles which will be applied to a set of inter-related genes involved in DNA replication and response to DNA damage. Since very few mutations have been isolated for many of these genes, this research should define new functions in the DNA replication and DNA damage response pathways. Broader impacts: The scientific goals of this research project are closely intertwined with two broader impacts. First, the resulting mutations will be made widely available to the yeast community, with a particular focus on incorporation of these new reagents into genome-wide systems analysis. Genetic networks constructed from separation-of-function missense mutations are likely to uncover previously unappreciated interfaces that were missed in prior systems analysis which employed currently available mutant strains. Second, this project will rely heavily on entry-level researchers (undergraduate and high school students) who will be responsible for generating the panels of separation-of-function mutations. This experience will allow very junior researchers to play a central role in a significant research project and also introduce them to the critical function that mentoring plays in the biomedical research community.

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Victoria Lundblad其他文献

Assembly and regulation of telomerase.
端粒酶的组装和调节。
The end replication problem: More than one solution
末端复制问题:不止一种解决方案
  • DOI:
    10.1038/nm1197-1198
  • 发表时间:
    1997-11-01
  • 期刊:
  • 影响因子:
    50.000
  • 作者:
    Victoria Lundblad
  • 通讯作者:
    Victoria Lundblad
Telomeres: has cancer's Achilles' heel been exposed?
端粒:癌症的致命弱点是否已被暴露?
  • DOI:
    10.1038/13451
  • 发表时间:
    1999-10-01
  • 期刊:
  • 影响因子:
    50.000
  • 作者:
    Louis A Zumstein;Victoria Lundblad
  • 通讯作者:
    Victoria Lundblad
A tale of ends
《终结之章》
  • DOI:
    10.1038/35003085
  • 发表时间:
    2000-01-13
  • 期刊:
  • 影响因子:
    48.500
  • 作者:
    Victoria Lundblad
  • 通讯作者:
    Victoria Lundblad

Victoria Lundblad的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Victoria Lundblad', 18)}}的其他基金

FASEB-sponsored Research Conference on Yeast Chromosome Structure being held in Snowmass Village, Colorado on August 19-24, 2000.
FASEB 赞助的酵母染色体结构研究会议于 2000 年 8 月 19 日至 24 日在科罗拉多州斯诺马斯村举行。
  • 批准号:
    0095241
  • 财政年份:
    2000
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant

相似海外基金

New substrates and functions for the DYRK protein kinases
DYRK 蛋白激酶的新底物和功能
  • 批准号:
    2894877
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Studentship
New substrates and functions for the DYRK protein kinases
DYRK 蛋白激酶的新底物和功能
  • 批准号:
    BB/Y512527/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Training Grant
Theory and algorithms for a new class of computationally amenable nonconvex functions
一类新的可计算非凸函数的理论和算法
  • 批准号:
    2416250
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
CAREER: New directions in the study of zeros and moments of L-functions
职业:L 函数零点和矩研究的新方向
  • 批准号:
    2339274
  • 财政年份:
    2024
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Elucidation of Characteristics and Functions of Rituals of Japanese New Religions : To Reconsider Their Worldviews and Hierarchies
日本新宗教仪式的特征和功能的阐释:重新考虑他们的世界观和等级制度
  • 批准号:
    22KJ1273
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Theory and algorithms for a new class of computationally amenable nonconvex functions
一类新的可计算非凸函数的理论和算法
  • 批准号:
    2309729
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Standard Grant
CAREER: New Challenges in Analysis of Boolean Functions
职业:布尔函数分析的新挑战
  • 批准号:
    2239160
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Continuing Grant
Exploring new functions of plastid-localized thioredoxin-like proteins
探索质体定位的硫氧还蛋白样蛋白的新功能
  • 批准号:
    23KJ0887
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Development of a new oral function training method focusing on human brain functions and its effectiveness
关注人脑功能的口腔功能训练新方法的开发及其有效性
  • 批准号:
    23K09253
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Discovery of new metabolic functions in Plasmodium parasites
发现疟原虫寄生虫的新代谢功能
  • 批准号:
    FT220100564
  • 财政年份:
    2023
  • 资助金额:
    $ 30万
  • 项目类别:
    ARC Future Fellowships
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了