FLP PROTEIN COMPLEXED W/ DNA

Flp是λ整合酶家族的位点特异性重组酶。 它与特定的DNA位点结合，然后切断DNA链， 在重组构型中重新连接它们，如下所示： 反应机理与Cre重组酶（1）非常相似， 这两种蛋白质之间的序列同源性非常有限。 FLP (from酵母）是该家族的唯一真核成员， 预计将在几个有趣的方面不同于综合招聘考试，包括 在组装活性位点中使用结构域交换。 的 重组反应不需要辅因子，并且重组反应的能量 DNA骨架的磷酸二酯键以共价键形式储存， 磷酸酪氨酸中间体。 在BioCARS Station上收集数据 14-BM-D

Flp is a site-specific recombinase of the lambda integrase family. It binds to specific DNA sites, then cuts the DNA strands and religates them in recombinant configuration, as below: Although the reaction mechanism is very similar to that of Cre recombinase (1), the sequence homology between the two proteins is extremely limited. Flp (from yeast) is the only eukaryotic member of this family and is expected to differ from cre in several intersting aspects, including the use of domain swapping in assembling the active site. The recombination reaction requires no cofactors, and the energy of the phosphodiester bonds of the DNA backbone is stored in a covalent phospo-tyrosine intermediate. Data was collected on BioCARS Station 14-BM-D.