CRYOBIOPHYSICAL CHARACTERISTICS OF SPHEROIDS

描述：球状体的冷冻特性是独特的， 与构成聚集体的分离细胞的不同。 的 低温性能的表征将是必不可少的， 球状体冷冻保存方案的开发。 两个不同 将研究感兴趣的实验系统：由 肝细胞和前列腺肿瘤细胞。 之所以选择这两个系统，是因为 他们的临床意义。 肝细胞球状体目前处于 用于生物人工肝的研究。 前列腺球状体有 以前被用来测试不同治疗的有效性 前列腺癌的治疗方法 本次调查的具体目的是：（1）确定基本的 形成聚集体的分离细胞的低温生物物理参数;（2） 为了确定球状体的基本低温生物物理参数， 冷冻;（3）建立单室和多室模型， 球体内的水输送;（4）使用 在不同的热和化学条件下的受控速率冷冻机， 确定球体内活细胞的空间分布， （5）开发提高生存能力的方法， 肝细胞球体和肿瘤球体在冻存过程中的损伤。 这一调查结果将为今后的研究提供合理的依据。 冷冻保存的冷冻方案的发展以及 冷冻手术方案。 肝细胞球冻存成功 将促进基于球体的肝脏支持的临床应用 系统. 确定损伤机理和增强方法 损伤将促进冷冻手术方案的发展， 前列腺癌的治疗

DESCRIPTION: The freezing characteristics of spheroids are unique and differ from that of the isolated cells which comprise the aggregate. The characterization of the low temperature properties will be essential to the development of cryopreservation protocols for spheroids. Two different experimental systems of interest will be investigated: spheroids made from hepatocytes and prostate tumor cells. These two systems were chosen because of their clinical relevance. Hepatocyte spheroids are presently under investigation for use in a Bio-Artificial Liver. Prostate spheroids have been used previously to test the effectiveness of different treatment modalities on prostate cancer. The specific aims of this investigation are: (1) to determine the basic cryobiophysical parameters of isolated cells which form the aggregates; (2) to determine the basic cryobiophysical parameters for the spheroids during freezing; (3) to develop single as well as multicompartment models for the water transport within a spheroid; (4) to freeze spheroids using a controlled rate freezer under different thermal and chemical conditions and determine the spatial distribution of viable cells within the spheroid and functional viability; (5) to develop methods of enhancing viability for hepatocyte spheroids and damage for tumor spheroids during cryopreservation. The results of this investigation will provide a rational basis for the development of freezing protocols for cryopreservation as well as cryosurgical protocols. Successful cryopreservation of hepatocyte spheroids will facilitate the clinical applications of spheroid-based liver support systems. Determination of the damage mechanisms and methods to enhance damage will facilitate development of cryosurgical protocols for the treatment of prostate cancer.