Multicomponent solutions for the preservation of cell therapy products

用于保存细胞治疗产品的多组分解决方案

基本信息

  • 批准号:
    10636645
  • 负责人:
  • 金额:
    $ 38.6万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2017
  • 资助国家:
    美国
  • 起止时间:
    2017-08-03 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

Project Abstract A novel, biologically inspired strategy will be used to improve DMSO-free preservation of cardiomyocytes derived from human induced pluripotent stem (hiPS) cells cultured as aggregates. Multicellular systems respond poorly to conventional preservation methods. In the previous funding cycle, a differential evolution algorithm was used to optimize the preservation of cells using combinations of sugars, sugar alcohols and amino acids. This approach mimicked the strategies of plants and other simple organisms that survive environmental stresses. Studies characterizing the behavior of multicomponent osmolyte solutions demonstrated that osmolytes present in these solutions interact with each other to modify the behavior of water during freezing which manifests itself at a molecular level (hydrogen bonding) as well as changes in microstructure. The osmolytes also interact with critical biological structures in the cell to stabilize them. Low temperature Raman spectroscopy studies demonstrated that multicellular systems are sensitive to undercooling (the temperature difference between the freezing temperature and the temperature at which ice forms in the extracellular solution). As we transition from single cells to aggregates, once again, we are looking to nature for inspiration. The wood frog uses combinations of osmolytes, suppressed cell metabolism, and minimizes undercooling as it adapts to freezing conditions in the winter. We are proposing a similar approach to improving preservation of cardiomyocyte aggregates. The studies described in the application characterize the freezing response of iPSC-derived cardiomyocytes at the committed cardiac progenitor stage and when formed into fully differentiated, multicellular cardiac constructs. The influence of undercooling on post thaw recovery of fully differentiated multicellular cardiac constructs will also be determined. The influence of suppressing cell metabolism on post thaw recovery of multicellular constructs will also be quantified. On a fundamental level, these studies will advance our understanding of the freezing behavior of more complex multicellular systems. On an applied level, the proposed investigation has the potential to preservation of cells differentiated from hiPS cells through the use of naturally inspired strategies.
项目摘要

项目成果

期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Comparative analysis of cell therapy infusion workflows at clinical sites.
  • DOI:
    10.1016/j.jcyt.2020.12.008
  • 发表时间:
    2021-04
  • 期刊:
  • 影响因子:
    4.5
  • 作者:
    Joules, Adam;Connors, Julianne;Johnson, Rachel;Van Orsow, Elizabeth A.;McKenna, David H.;Nikiforow, Sarah;Ritz, Jerome;Gee, Adrian;Hubel, Allison
  • 通讯作者:
    Hubel, Allison
Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.
Understanding the freezing responses of T cells and other subsets of human peripheral blood mononuclear cells using DSMO-free cryoprotectants.
  • DOI:
    10.1016/j.jcyt.2020.01.013
  • 发表时间:
    2020-05
  • 期刊:
  • 影响因子:
    4.5
  • 作者:
    Pi CH;Hornberger K;Dosa P;Hubel A
  • 通讯作者:
    Hubel A
Natural deep eutectic systems for nature-inspired cryopreservation of cells.
天然深共晶系统,用于受自然启发的细胞冷冻保存。
Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.
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ALLISON HUBEL其他文献

ALLISON HUBEL的其他文献

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{{ truncateString('ALLISON HUBEL', 18)}}的其他基金

Multicomponent solutions for the preservation of cell therapy products
用于保存细胞治疗产品的多组分解决方案
  • 批准号:
    10439717
  • 财政年份:
    2017
  • 资助金额:
    $ 38.6万
  • 项目类别:
Multicomponent solutions for the preservation of cell therapy products
用于保存细胞治疗产品的多组分解决方案
  • 批准号:
    10200145
  • 财政年份:
    2017
  • 资助金额:
    $ 38.6万
  • 项目类别:
Integrated training in development and clinical practice of cell-based therapies
细胞疗法开发和临床实践的综合培训
  • 批准号:
    9127354
  • 财政年份:
    2015
  • 资助金额:
    $ 38.6万
  • 项目类别:
Integrated training in development and clinical practice of cell-based therapies
细胞疗法开发和临床实践的综合培训
  • 批准号:
    9487293
  • 财政年份:
    2015
  • 资助金额:
    $ 38.6万
  • 项目类别:
Integrated training in development and clinical practice of cell-based therapies
细胞疗法开发和临床实践的综合培训
  • 批准号:
    9283633
  • 财政年份:
    2015
  • 资助金额:
    $ 38.6万
  • 项目类别:
Technology platform for development of multi-component preservation solution
多组分保存解决方案开发技术平台
  • 批准号:
    8582171
  • 财政年份:
    2013
  • 资助金额:
    $ 38.6万
  • 项目类别:
Technology platform for development of multi-component preservation solution
多组分保存解决方案开发技术平台
  • 批准号:
    8703695
  • 财政年份:
    2013
  • 资助金额:
    $ 38.6万
  • 项目类别:
Post thaw assessment of umbilical cord blood
脐带血解冻后评估
  • 批准号:
    8457137
  • 财政年份:
    2012
  • 资助金额:
    $ 38.6万
  • 项目类别:
Post thaw assessment of umbilical cord blood
脐带血解冻后评估
  • 批准号:
    8261833
  • 财政年份:
    2012
  • 资助金额:
    $ 38.6万
  • 项目类别:
Cell Graft Engineering Using Microfluids
使用微流体的细胞移植工程
  • 批准号:
    7229928
  • 财政年份:
    2006
  • 资助金额:
    $ 38.6万
  • 项目类别:

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