REGULATION OF MYOMETRIAL CYCLASE ISOTYPE EXPRESSION

描述：（改编自申请人的摘要）确切的机制 导致子宫张力增加，从而导致早产或子宫 张力亢进尚未阐明。 最重要的生产途径 子宫收缩和舒张是OT和bAR介导的途径。 的 该提案的目标是了解这两者之间的相互作用， 非妊娠子宫肌层中的通路，妊娠如何影响这种相互作用 以及通路之间的相互作用如何影响对bAR变化的反应 活动 这些研究将在新鲜和培养的孕妇中进行。 和非妊娠大鼠和人子宫肌层，用OT预处理。 研究将 包括测量bAR和OT受体以及AC数量和亲和力 活动 为了确定AC的特定亚型的作用，他们将 确定这些亚型的蛋白质和mRNA的存在和数量 参与AC的脱敏。 相互作用的知识 导致子宫过度活跃的信号传导途径之间的联系 用于预防胎儿窘迫、早产和子宫张力过高。

DESCRIPTION: (Adapted from the applicant's abstract) The exact mechanisms leading to increased uterine tone resulting in preterm labor or uterine hypertonus have not been elucidated. The most important pathways producing uterine contraction and relaxation are OT and bAR-mediated pathways. The goals of the proposal are to understand the interactions between these two pathways in non pregnant myometrium, how pregnancy affects this interaction and how interaction between pathways affects responses to changes in bAR activity. These studies will be performed in a fresh and cultured pregnant and non pregnant rat and human myometrium, pretreated with OT. Studies will include measurement of numbers and affinities of bAR and OT receptors and AC activity. To identify the role of specific isoforms of AC, they will determine presence and quantities of protein and mRNA for those isoforms involved in the desensitization of AC. The knowledge of the interaction between signal transducing pathways causing uterine hyperactivity will lead to prevention of fetal distress and preterm labor and uterine hypertonus.