RNA TRANSPORT IN DENDRITES

神经细胞的描述(从申请人的摘要中修改)，受监管 树突中一些关键突触后蛋白的局部合成可能 对长期结构和功能具有至关重要的相关性 突触连接的可塑性。这样的一个必要的先决条件 其机制是将选定的RNA靶向转移到树突部位 翻译。树突中特定的RNA运输的概念已经获得 申请人实验室中最近观察到的顺式代理支持 含有树突状RNA的信号元件负责 将核糖核酸定位于树突。根据这些数据， 本研究项目的中心目标被定义为解剖 核糖核酸在树突中的传输机制及反馈分析 允许树突状RNA的局部调节的机制 通过突触的生理事件进行运输。在特定目标中1 计划以主要和/或更高的顺序定义 结构，最小本质目标的物理精细结构 模块(S)。在第二个目标中，将确定是否以及如何 树突状RNA的运输是通过神经元活动来调节的。在……下面 这一具体目标是什么机制正在被使用的问题 神经元对树突状细胞蛋白多样性的调节 突触的功能性输入。这项研究项目的总体目标是 从而确定树突状RNA的功能意义 运输机制很可能在 对马赛克突触后蛋白谱系的调节，从而对 神经间突触连接的发育和长期可塑性 细胞。

DESCRIPTION (Modified from applicant's abstract) In nerve cells, regulated local synthesis in dendrites of a number of key postsynaptic proteins may be of critical relevance for the long-term structural and functional plasticity of synaptic connections. An essential prerequisite for such a mechanism is the targeted transport of select RNAs to dendritic sites of translation. The notion of specific RNA transport in dendrites has gained support by the recent observation in the applicant's lab that a cis-acting signaling element that is contained with a dendritic RNA is responsible for the targeting of the RNA to dendrites. Based on these data, the central objectives of this research project are defined as the dissection of RNA transport mechanisms in dendrites and the analysis of feedback mechanisms that would allow the local regulation of dendritic RNA transport through physiological events at the synapse. In Specific Aim 1 it is planned to define, in terms of primary and/or higher order structure, the physical fine structure of minimal essential targeting module(s). In the Second Aim, it will be established whether and how transport of dendritic RNAs is regulated through neuronal activity. Under this Specific Aim is the question of what mechanisms are being used by neurons to modulate the diversity of dendritic proteins in response to functional input at the synapse. The overall goal of this research project is thus to ascertain the functional significance of dendritic RNA transport mechanisms that are likely to be of vital importance in the regulation of mosaic postsynaptic protein repertoires, and thus for the development and long-term plasticity of synaptic connections between nerve cells.