NOVEL HUMAN ORAL & CRANIOFACIAL GENES

本合同的目的是发现和开始表征新的人类基因表达优先在胚胎菱形和第一鳃弓，口腔（包括牙齿，唾液腺和舌头），和上颌骨。 它将提供与口腔和颅面遗传学和发育有关的新数据和人类DNA克隆的国家资源。 具体目标是：1.产生一组准确分期的组织特异性和发育阶段特异性的人类颅面cDNA文库，并提供有关组织（来源和质量）、胚胎阶段和文库质量的文件。2.标准、标准化、消减和SAGE文库应保存在公共储存库中，例如由ATCC或I.M.A.G.E.管理。联合体，或者也可以提供给NIDCR项目官员。 此外，还应向I.M.A.G.E.提供涵盖人类口腔和颅面发育许多早期阶段的cDNA文库。传统EST生成的联盟。3.应用筛选方法来比较在不同的人类颅面胚胎阶段或组织中表达的基因，以产生该领域的研究人员可以用来获得和表征发育阶段特异性和组织特异性的cDNA克隆的信息。4.鉴定和分离阶段特异性（特别是早期阶段）或异常高表达的组织特异性克隆。5.本合同产生的信息，包括DNA序列和数据库检索摘要，应及时公开。 当数据通过存款在网站上可用时，应公开发布数据。

The purpose of this contract is to discover and to begin to characterize novel human genes expressed preferentially in embryonic rhombomeres and first branchial arch, oral cavity (including teeth, salivary glands and tongue), and maxilla. It will provide a national resource of novel data and human DNA clones relevant to oral and craniofacial genetics and development. The specific goals are: 1. Produce a set of accurately staged tissue-specific and developmental stage-specific human craniofacial cDNA libraries, with documentation concerning the tissue (source and quality), embryonic stage, and quality of the library. 2. The standard, normalized, subtracted, and SAGE libraries shall be deposited in a public repository such as managed by the ATCC or I.M.A.G.E. consortium, or they can alternatively be provided to the NIDCR Project Officers. In addition, cDNA libraries covering many of the early stages of human oral and craniofacial development shall be provided to the I.M.A.G.E. consortium for conventional EST generation. 3. Apply screening approaches to compare the genes expressed in the different human craniofacial embryonic stages or tissues, in order to generate information that researchers in the field can use to obtain and characterize cDNA clones that are developmental stage-specific and tissue-specific. 4. Identify and isolate stage-specific (particularly from early stages) or unusually highly expressed tissue-specific clones. 5. Information generated by this contract, including DNA sequences and summaries of database searches, shall be made publicly available promptly. There should be open release of data as it becomes available by deposit in a web site.