PHYSIOLOGY OF THE DEVELOPING OLFACTORY EPITHELIUM

描述：(改编自申请者摘要)嗅觉提供 对非常年轻的人来说是至关重要的信息。嗅觉线索引导新生儿 喂养并允许兄弟姐妹从其他同种生物中识别出来。 母体或环境线索上的印记发生在生命的早期，当 嗅觉系统正在迅速发展。无数的，解剖学的， 免疫细胞化学和分子遗传学研究已经取得进展 了解早期发展过程中发生的变化。 使用活动依赖标记的生理学研究已经证明 嗅球和高级嗅觉中枢在中枢神经系统中的重要作用 偏好学习和印记。然而，几乎没有什么信息。 关于嗅觉上皮的基本生理发育 在偏好学习或印记过程中可能发生的变化。我们 对发育中的嗅觉上皮进行功能表征的建议 使用常规电生理和钙离子对斑马鱼的影响 成像方法和一种新的活性依赖免疫标记技术。 这种新兴的发展模式之所以被选中，是因为它孕育了一年- 轮，受精和发育发生在外部和迅速和 可以维持大量的时间阶段胚胎。我们的首要目标是 以确定受精后的电压、钙和秒 传递气味结合事件所需的信使门控电流 变成了一种行动潜力的开发。我们的第二个和第三个目标是 气味敏感性的时间发展和空间分布 嗅觉刺激的嗅觉感受器神经元(ONs)。气味刺激角鸟 将标记有离子通道的探针，1-氨基-4- 胍基丁烷(AGB)和抗AGB抗体。互补性 所研究的电生理和钙成像将表征 原地和个体发育卵的反应特性。决赛 目的是确定气味环境在功能上所起的作用 嗅觉上皮的发育。建议的研究将会有所帮助。 确定在功能发展的关键时期 并可能提供对嗅觉上皮细胞相关作用的洞察 正常发育中的环境与程序性遗传事件 流程。

DESCRIPTION: (Adapted From the Applicant's Abstract) Olfaction provides critical information to the very young. Olfactory cues guide neonates to feed and permit siblings to be recognized from other conspecifics. Imprinting on maternal or environmental cues occurs early in life when the olfactory system is rapidly developing. Numerous, anatomical, immunocytochemical and molecular genetic studies have advanced out understanding of the changes occurring during early development. Physiological studies, using activity dependent markers, have demonstrated the essential roles of the olfactory bulb and higher olfactory centers in preference learning and imprinting. There is, however, little information about the basic physiological development of the olfactory epithelium or the changes that might occur during preference learning or imprinting. We propose to functionally characterize the developing olfactory epithelium of the zebrafish using conventional electrophysiological and calcium imaging methods and a novel activity-dependent immunolabeling technique. This emerging developmental model was selected because it breeds year- round, fertilization and development occur externally and rapidly and large numbers of time-staged embryos can be maintained. Our first aim is to determine when after fertilization the voltage, calcium and second messenger-gated currents required to transduce an odorant-binding event into an action potential develop. Our second and third aims characterize the temporal development of odor sensitivity and the spatial distribution of odor-stimulated olfactory receptor neurons (ORNs). Odor-stimulated ORNs will be labeled with an ion channel permeant probe, 1-amino-4- guanidobutane (AGB), and anti-AGB IgG antibodies. Complementary electrophysiological and calcium imaging studied will characterize the response properties of developing ORNs in situ and individually. The final aim is to determine the role odor environment play in the functional development of the olfactory epithelium. The studies proposed will help identify the critical periods in the functional development of the olfactory epithelium and may provide insight into the relative roles of environment versus programmed genetic events in normal developmental processes.