MOLECULAR STRUCTURE OF ENDOSOMES IN DEVELOPING INTESTINE

描述：这是一份继续学习的续期申请 老年人胃肠道吸收功能的发展 新生大鼠。新生儿早期的肠细胞有一种详细的 具有吞噬能力的内涵体系统增加了 大分子。肠道也有吞噬这些物质的能力。 有选择地运输生长等生理分子的囊泡 因子和荷尔蒙通过细胞而不受干扰 直接贴在细胞上或从基侧膜完整地延伸出来。 或者，肠细胞可以起到粘膜屏障的作用，以防止 对病理性抗原和微生物的吸收。校长 研究人员已经确定了一种名为内皮管素的分子，它是一种特定的 存在于发育中的肠道顶端内体内的蛋白质和 可以作为一个标记来定义顶端内体的功能。 并确定肠小体的发育过程 在胃肠道内的功能。这股潮流的目标是 建议，已经克隆了内管素DNA并确定了其对 非极化和极化单元，用于：1)识别目标信号 参与特化内体的生物发生。2)定义 通过这个隔间进行内吞和循环。3)隔离新的 内体蛋白。4)绘制人类染色体定位图 人类基因组中的内皮管酶基因。如果实现了这些目标，一个 更好地理解上皮作为一种屏障的重要性 管腔环境即将到来和使用内窥体的方法 在抗体或抗体的传递中适当的贩运模式 可以更好地理解生理分子。

DESCRIPTION: This is a renewal application to continue to study the development of absorptive function within the gastrointestinal tract in the neonatal rat. The enterocyte during early neonatal period has an elaborate endosomal system that has the capacity to endocytosis increase quantities of macromolecules. The intestinal tract also has a capacity for these engulfed vesicles to selectively transport physiologic molecules such as growth factors and hormones through the cell without disruption and either act directly on the cell or extended intact from the basolateral membrane. Alternatively, the enterocyte can function as a mucosal barrier to prevent the uptake of pathologic antigens and micro-organisms. The principal investigator has identified a molecule, endotubin, which is a specific protein present in the developing intestine within the apical endosome and can be used as a marker to define the function of apical endosomes in the developing intestine and to determine the process by which the endosome functions within the gastrointestinal tract. The goals of this current proposal, having cloned the endotubin DNA and identified its effect on non-polarized and polarized cell, is to: 1) identify the targeting signals involved in the biogenesis of specialized endosomes. 2) to define endocytosis and recycling through this compartment. 3) to isolate new endosomal proteins. 4) to map the chromosome localization of the human endotubin gene within the human genome. If these goals are accomplished, a better understanding of the importance of the epithelium as a barrier to the luminal environment will be forthcoming and ways to use the endosomal trafficking pattern appropriately in the delivery of either antibodies or physiologic molecules can be better understood.