REGULATION OF ICTACALCIN GENE EXPRESSION

描述(改编自申请人的摘要)：ICTCALIN是一种 在嗅觉感受器神经元中发现的钙结合蛋白 那条鲶鱼。这一领域的提案描述了一系列旨在 ICTCALIN基因及其机制的研究 仅限于嗅觉神经元表达。嗅觉信号 脊椎动物中通过G蛋白偶联介导的转导 途径，最终导致细胞内钙离子的波动 受体神经元的纤毛和胞体。因此，钙被认为是 参与信号产生、适配和/或终止 感官信号。ICTCALIN属于钙结合蛋白S100家族 蛋白质，其成员可能在调节效应中发挥作用 钙在各种其他组织中的含量。因为它具有组织特异性 ICTCALIN在嗅觉上皮细胞中的表达及调控 吉恩可能是个有趣的人。在这份申请中，波塔博士建议 分离ICTCALIN全长基因并对其进行定义 其调控区域和细胞类型的转录元件 参与其调控的特定转录因子。在 从长远来看，P.I.认为这项工作对 了解化学感觉转导的调节及其他 神经突起。

DESCRIPTION (adapted from applicant's abstract): Ictacalcin is a calcium-binding protein that is found in olfactory receptor neurons in the catfish. This AREA proposal describes a series of experiments aimed at characterizing the ictacalcin gene and the mechanisms underlying its restricted expression to olfactory neurons. Olfactory signal transduction in vertebrates, mediated through G protein coupled pathways, results eventually in fluctuations of cytosolic calcium in the cilia and soma of receptor neurons. Thus, calcium is thought to be involved in signal generation, adaptation and/or termination of the sensory signal. Ictacalcin belongs to the S100 family of calcium binding proteins, the members of which may play a role in mediating the effects of calcium in a variety of other tissues. Because of its tissue-specific expression in the olfactory epithelium, the regulation of the ictacalcin gene is potentially interesting. In this application, Dr. Porta proposes to isolate the full-length ictacalcin gene and initiate a definition of transcriptional elements in its regulatory regions and of cell type specific transcriptional factors involved in its regulation. In the long-term, the P.I. believes that this work has significance for understanding the regulation of chemosensory transduction and other neuronal processes.