YOLK SAC STEM CELL DIFFERENTIATION IN VITRO AND VIVO

描述(改编自调查人员的摘要)：假设 这种应用是在体外扩增卵黄囊造血干细胞 可以成功地移植到组织不相容的宿主中，在这些宿主中 分化，从而建立免疫功能。具体目标是 为：1)创造条件促进卵黄囊来源的自我更新 体外培养小鼠造血干细胞；2)测定小鼠骨髓间充质干细胞 扩增卵黄囊来源干细胞群(A)移植小鼠靶器官 在组织相容的宿主动物中；(B)产生各种血细胞 血统；以及(C)产生能够在免疫中发挥作用的细胞 系统；以及3)确定扩增的卵黄囊来源的干细胞 种群还可以播种小鼠的靶器官，并在 组织不相容的宿主动物。

DESCRIPTION (Adapted from the investigator's abstract): The hypothesis of this application is that in vitro expanded yolk sac hematopoietic stem cells can successfully engraft in histoincompatible hosts in which they then differentiate, thereby establishing immune functions. The specific aims are to: 1) establish conditions that promote self-renewal of yolk sac-derived mouse hematopoietic stem cells in vitro; 2) determine the ability of the expanded yolk sac-derived stem population (a) to seed murine target organs in histocompatible host animals; (b) to generate the various blood cell lineages; and (c) to yield cells capable of functioning in the immune system; and 3) determine whether the expanded yolk sac-derived stem cell population can also seed murine target organs and differentiate in histoincompatible host animals.