METALLOENZYMES AND COLLAGEN METABOLISM

这项研究的目的是要探讨其作用机制。 胶原酶的作用，胶原酶的独特能力是催化 生理条件下胶原蛋白的降解。胶原蛋白， 结缔组织的主要结构蛋白，对 被所有已知的消化酶和细胞内酶攻击。因此， 胶原酶的作用是所有过程中必不可少的涉及 结缔组织的降解或重塑。特别是，他们有 与伴随而来的酶关节破坏有关 关节炎。胶原酶将根据不同的来源进行分离 公布的程序和通过亲和层析的方法 发展起来的。将开发新的快速荧光分析方法 以加快分离和纯化步骤。所获得的酶 将根据它们的同质性、金属含量、 氨基酸组成、相对分子质量和亚基结构。角色 金属原子(S)在催化中的作用将通过检查 金属取代离子对酶活性的影响。化学改性 将进行反应以识别必需的氨基酸残基。 这项研究应该会促进人们更好地理解 哺乳动物结缔组织代谢中的胶原酶。

The objective of the proposed research is to investigate the mechanism of action of collagenases, which are unique in their ability to catalyze the degradatio nof collagen under physiological conditions. Collagen, the major structural protein of connective tissue, is highly resistant to tattack by all known digestive and intracellular enzymes. Hence, the action of collagenase is essential to all processes involving the degradation or remodelling of connective tissues. In particular, they have been implicated in the enzymatic joint destructon that accompanies arthritis. Collagenases will be isolated from various sources according to published procedures and by affinity chromatographic methods to be developed. New and rapid fluorometric assay procedures will be developed to expedite the isolation and purification steps. The enzymes obtained will be characterized with respect to their homogeneity, metal content, amino acid composition, molecular weight and subunit structure. The role of the metal atom(s) in catalysis will be investigated by examining the effect of metal substituions on enzymatic activity. Chemical modificatin reactions will be carried out to identify essential amino acid residues. This research should promote a greater understanding of the role of collagenases in mammalian connective tissue metabolism.